免疫抑制酸性蛋白单克隆抗体MI2体外抗肿瘤作用的观察

作者采用ＭＴＴ比色法观察了本室自制免疫抑制酸性蛋白单克隆抗体ＭＩ＿２的体外抗肿瘤作用。结果表明：ＭＩ＿２在浓度为７．８１ｍｇ／Ｌ时即可明显抑制胃癌细胞株ＳＧＣ７９０１的生长（抑制率７．５％±２．４％Ｐ＜０．０１），并见剂量依赖性表现；在ＬＡＫ细胞与ＳＧＣ７９０１胃癌细胞的效靶比为１０：１时，加入１．９５ｍｇ／Ｌ的ＭＩ＿２即可明显增强ＬＡＫ细胞的细胞毒作用（增强效应为２０６．３％Ｐ＜０．０１），其作用也呈剂量依赖性表现。作者还发现ＭＩ＿２的这种抗肿瘤作用与补体无关。

ANTITUMOR ACTIVITY OF MONOCLONAL ANTIBODY MI_2 AGAINST IMMUNOSUPPRESSIVE ACIDIC PROTEIN IN VITRO

In vitro antitumor actjvjty of monoclonal antibody MI_2 that was made by our laboratory to direct against immunosuppressive acidic protein(IAP) was observed with MTT assay for cytotoxicity. The re-sults showed that the growth of human gastric cancer cell line SGC 7901 was inhibited significantly(P<0.01)when MI_2 was added at a concentration of 7.81mg/ L or higher. The inhibition activity of MI_2 appeared to be dose dependent.Increased cytotoxicity(up to 206.3%)of LAK cells against SGC7901 could be remarkably(P<0.01) induced by addition of MI_2 at a coneentration of 1.95mg / L,so the ratio of effector to target was 10:1. The enhancing effect of MI_2 on LAK cell activity was also dose dependent.The antitumor activity of MI_2 was not associated with human complements.