前列腺素E1治疗实验性肝坏死的机理研究

应用丙酸杆菌和内毒素引起肝坏死动物模型对前列腺素Ｅ＿１（ＰＧＥ＿１）抑制肝坏死发生作用及其机理进行了研究。结果：①ＰＧＥ＿１投给组的动物生存率为９０％较对照组（１７％）明显增高。肝组织学检查未发现肝细胞坏死；②当ＰＧＥ＿１添加后。丙酸杆菌处理小白鼠肝内枯否细胞培养上清液中的肿瘤坏死因子（ＴＮＦ）、分泌型白细胞介素１（ＳＩＬ－１）和膜型白细胞介素１（ｍＩＬ－１）活性受到抑制，其抑制程度随ＰＧＥ＿１浓度增加而增大。上述结果说明：ＰＧＥ＿１可以抑制肝坏死的发生，其作用机制可能与抑制ＴＮＦ、ＳＩＬ－１和ｍＩＬ－１活性有关。

THE PROTECTIVE EFFECT OF PROSTAGLANDIN E_1 IN EXPERIMENTAL MODEL OF MASSIVE LIVE CELL NECROSIS

he protective effect of PGE_1 in mice injected with propionibacterium acnes(Pacnes)and subse-quently with a small dose of lipopoly-saccharide-endotoxin (LPS)7 days later was studied.The results of this study were as follows:(1)When PGE_1 was administered one hour before the injection of LPS,the survival rate of mice was significantly higher(90%),than that of a control group(17%).No necrosis was found in the liver.(2)PGE _1 suppressed the release of tumor necrosis factor(TNF)and secreted-interleukin-1(SIL-1)in culture supernatants of P.acnes-elicited kupffer cells and membrane IL-1(mIL-1)in kupffer cells in a dose-dependent manner in vitro. These results strongly suggest that PGE_1 can prevent liver cell necrosis in the animal model and the mechanism of curative effect of PGE_1 in massive liver cell necrosis may result from inhibition of the activity of TNF,sIL-1 and mIL-1 in the kupffer cells.