聚合酶链反应检测乙型肝炎患者肝外组织中的乙型肝炎病毒DNA

应用巢式聚合酶链（ＰＣＲ）技术对１８例石蜡包埋的胆囊、肾、脾、肾上腺、心、睾丸、胰腺及肝脏组织的乙型肝炎病毒（ＨＢＶ）ＤＮＡ进行了检测，并与其免疫组化及原位杂交方法作比较。二组引物检测的结果表明：肝组织中均有ＨＢＶ感染，其中５例有ＨＢＶ的复制。肝外组织中ＨＢＶ检出率分别为胆囊８５．７％、７５．０％、肾７２．７％、肾上腺６６．７％、心脏５５．６％、睾丸５５．６％和胰腺５４．５％，但均未检测出ＨＢＶ的复制，ＰＣＲ检测发现与免疫组化及原位杂交结果基本一致。ＨＢＶ可以感染肝外组织但并不在这些组织中复制的发现可以解释受感染的肝外组织可以作为肝外感染源，但并不引起这些脏器出现明显的病理变化。

DETECTION OF HBV DNA TN EXTRAHEPATIC TISSUE WITH PCR COMPARISON WITH IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION

ormalin-fixed paraffin-embedded tissue,including gallbladder,kidney,spleen,adrenal gland,heart,testicle,pancreas,nd liver from eighteen autopsied cases with HBV infection were studied with nested polymerase chain reaction (PCR) for detection of HBV DNA, The DNA sequence representing HBV infection was detected in the tissue of liver(100%),gallbladder(6 from7,85.7%),spleen(6 from 8,75.0%),kidney(8 from 11,72.7%)adrenal gland(4 form 6, 66.7%),heart(10 from 18,55.6%),testicle(10 from 18,55.6%),pancreas(6 from 11,54.5%) respectively. The DNA sequence representing HBV replication was detected in 5 cases of liver tissue only.The findings of PCR was correlated with the result of immunohistochemistry and in situ hybridization,It is shown that HBV can infect extrahepatic tissue but do not replicate in it We think these findings may explain that the harboring ofhepatitis virus in extrahepatic tissue could serve as one of extrahepatic infective sources, but have little pathological consequence on the infected extrahepatic organs.