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Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells |
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| Authors: | M. Stya R. Wahl W.H. Beierwaltes |
| Affiliation: | Division of Nuclear Medicine, Department of Internal Medicine University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. |
| Abstract: | Two assays are described that are suitable for the screening of large numbers of hybridoma supernatants as well as for the screening of a wide range of tissues for the distribution of a given cell surface antigen. These assays use whole live cells as targets, avoiding fixation or extraction, which could alter the antigenic structural profile. The assays are rapid, simple and inexpensive. Their advantages and applications are discussed. |
| Keywords: | hybridoma screening ELISA radioimmunoassay ELISA enzyme-linked immunosorbent assay RIA radioimmunoassay BSA bovine serum albumin PBS phosphate-buffered saline pH 7.2 SPA staphylococcal protein A |
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