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Plaque-forming cell response of frozen human lymphocytes stimulated with pokeweed mitogen. II. Results obtained in cultures of irradiated T and untreated B lymphocytes
Authors:Palle Tauris
Affiliation:Department of Clinical Immunology, The Tissue Typing Laboratory, Aarhus Kommunehospital, University of Aarhus, DK-8000 Aarhus C, Denmark
Abstract:
The PFC capability of cryopreserved lymphocytes was tested after T/B cell separation and irradiation of the T cells. It is concluded that there is no selective loss of function of the involved subpopulations. Thus nitrogen-stored lymphocytes can safely be used in PFC assays after conventional separation and irradiation procedures.
Keywords:plaque-forming cells  cryopreserved cells  isolated T and B lymphocytes  irradiated T cells
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