免疫球蛋白超家族黏附分子在淋巴管和不同血管内皮细胞的表达

目的比较免疫球蛋白超家族黏附分子在淋巴管、大血管和微血管内皮细胞的表达特点，探讨免疫球蛋白超家族黏附分子在淋巴管内皮细胞表达的意义。方法从狗的胸导管、颈总动脉、颈内静脉、肺微血管分离内皮细胞，利用免疫荧光标记法检测PECAM-1、ICAM-1、ICAM-3、VCAM-1和CD44在各种内皮细胞的表达，在荧光显微镜和激光共聚焦扫描显微镜下观察，并用图像分析仪分析表达强度。结果动脉、静脉和肺微血管内皮细胞表达PECAM—1、ICAM—1、ICAM-3、VCAM—1和CD44。其中，ICAM-1和ICAM-3的表达较弱。VCAM—1在动脉和肺微血管内皮细胞的表达比静脉强。淋巴管内皮细胞表达PECAM—1、ICAM—1、ICAM-3和CD44，未观察到VCAM—1的表达。ICAM-3和CD44的表达比血管内皮细胞强。结论与动脉、静脉和微血管内皮细胞比较，淋巴管内皮细胞不表达VCAM—1，而ICAM-3和CD44表达较强，这有助于解释淋巴细胞和肿瘤细胞与淋巴管内皮的黏附以及淋巴管新生的机制。

EXPRESSION OF ADHESION MOLECULES OF IMMUNOGLOBIN SUPER FAMILY ON LYMPHATIC ENDOTHELIAL CELLS AND DIFFERENT VASCULAR ENDOTHELIAL CELLS

Objective To compare features in expression of adhesion molecules of immunoglobin super family on lymphatic, vascular and microvascular endothelial cells and explore implications in expression of the adhesion molecules of immunoglobin super family on lymphatic endothelial cells. Methods Endothelial cells were isolated from canine thoracic ducts, common carotid arteries, internal jugular veins and pulmonary microvessels. Expression of PECAM-1, ICAM-1, ICAM-3, VCAM-1 and CD44 on all kinds of endothelial cells were examined by immunofluoreseenee staining and viewed using a fluorescence microscope and a confocal laser scanning microscope. Expression strength of the adhesion molecules was analyzed with an image analyzer. Results The arterial, venous and microvascular endothelial cells expressed PECAM-1, ICAM-1, ICAM-3, VCAM-1 and CD44. Expression of ICAM-I and ICAM-3 on the cells was weak. Expression of VCAM-1 on the arterial and microvascular endothelial cells was stronger than that on venous endothelial cells. The lymphatic endothelial cells expressed PECAM-1, ICAM-1, ICAM-3 and CD44, expression of VCAM-1 was not observed. Expression of ICAM-3 and CD44 on the cells was stronger than that on vascular and microvascular endothelial cells. Conclusion In comparison with the arterial, venous, microvascular endothelial cells, lymphatic endothelial cells do not express VCAM-1, expression of ICAM-3 and CD44 is stronger. This is helpful to explain mechanisms of adhesion of lymphocytes or tumor cells to the lymphatic endothelium and lymphangiogenesis.