| 大鼠急性脊髓损伤后神经胶质酸性蛋白的表达意义 |
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| 引用本文： | 李宽新,王维山,史晨辉.大鼠急性脊髓损伤后神经胶质酸性蛋白的表达意义[J].实用医学杂志,2011,27(22):4034-4036. |
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| 作者姓名： | 李宽新 王维山 史晨辉 |
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| 作者单位： | 石河子大学医学院一附院骨一科,832000 |
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| 摘 要： | 目的:探讨脊髓损伤后神经胶质酸性蛋白(GFAP)的表达及对后肢功能恢复的影响。方法:健康的SD大鼠30只,Allen’s法打击T9～T10脊髓节段,用BBB法评估后肢的运动功能,免疫组化染色和图像分析法观察GFAP的表达。结果:BBB评分和行为观察,发现大鼠脊髓损伤后有自行恢复率68%:对照组显示GFAP在脊髓各个部位均有表达;实验组脊髓损伤1d后,损伤区域GFAP表达增加,可达损伤区附近、软脊髓膜下的白质和脊髓中央管均有GFAP的表达,脊髓损伤3～5 d后,脊髓损伤区域和邻近的周边区域GFAP的表达显著增加,并逐渐达到高峰,随着时间的推移GFAP的表达逐渐下降,GFAP的表达于损伤2周后逐渐恢复到对照组水平(P>0.05)。结论:大鼠脊髓损伤可诱导星形胶质细胞增生,脊髓损伤后的微环境适合胶质细胞增生,对中枢系统损伤修复产生影响。
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| 关 键 词： | 脊髓损伤 BBB评分 GFAP |
Expression of glial fibrillary acidic protein after acute spinal cord injury in rats |
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| LI Kuan-xin,WANG Wei-shan,SHI Chen-hui.Expression of glial fibrillary acidic protein after acute spinal cord injury in rats[J].The Journal of Practical Medicine,2011,27(22):4034-4036. |
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| Authors: | LI Kuan-xin WANG Wei-shan SHI Chen-hui |
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| Institution: | LI Kuan-xin,WANG Wei-shan,SHI Chen-hui.Department of Orthopaedics,the First Affiliated Hospital of the Medical College,Shihezi University,Shihezi 832000,China |
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| Abstract: | Objective To investigate the expression of glial fibrillary acidic protein(GFAP) and its effect on the recovery of hind limb function after spinal cord injury(SCI) in rats.Methods Thirty rats were randomly divided into blank control group(n=5) and injury group(n=25).Spinal cord was injured with Allen's weight dropping model,and each rat received contusion on its spinal cord at T9 with modified Allen's impactor.The motor function was evaluated by the Basso,Beattie & Bresnahanlocomotor rating scale(BBB scale)... |
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| Keywords: | Spinal cord injury BBB scale GFAP |
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