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Fast determination of retinol and alpha-tocopherol in plasma by LC
Authors:Rodríguez-Delgado M A  Díaz-Flores Estévez J F  Díaz-Flores Estévez F  Hernández Calzadilla C  Díaz Romero C
Affiliation:Department of Analytical Chemistry, Nutrition and Food Science, University of La Laguna, 38201, La Laguna, Spain. mrguez@ull.es
Abstract:
A fast, selective and economical method for the determination of retinol and alpha-tocopherol is presented. Both vitamins are separated by high-performance liquid chromatography (HPLC) in less than 4 min using an isocratic elution with methanol. The robustness of the method was checked in real samples, obtaining relative standard deviation lower than 3%. The described method was satisfactorily applied to serum samples proceeding of patients enrolled in a METHADONE maintenance treatment program. The retinol concentrations in the serum of these patients fell into the normal interval of concentrations; however, the serum alpha-tocopherol contents were higher than the normal values.
Keywords:
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