A modified approach to identification of the sickle cell anemia mutation by means of allele-specific polymerase chain reaction. |
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Authors: | K R Birikh O V Plutalov E I Schwartz P S Devi Y A Berlin |
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Institution: | M.M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. |
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Abstract: | The allele-specific PCR approach has been modified by introducing a second mismatch at the 3'-penultimate link of the primer and used to identify the sickle cell anemia mutation (A-->T transversion in the sixth codon of the human beta-globin gene causing Glu-->Val substitution in the protein), thus obviating the problem of an interpretationally ambiguous 3'-terminal mismatch including T residue. |
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Keywords: | Genomic DNA Point mutations Primers 3′-penultimate mismatch |
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