光动力疗法联合EDTA进行根管消毒的体外研究

目的：探究光动力疗法（photodynamictherapy,PDT）与EDTA联合应用进行根管消毒的效果。方法：选取75颗人单根管离体前磨牙建立感染根管模型,随机均分为5组,A组：PDT处理组;B组：EDTA处理组;C组：PDT联合EDTA处理组;D组：NaCIO阳性对照组;E组：生理盐水阴性对照组。各组处理前、处理后分别用纸尖在根管内取样,进行平板菌落计数。回复实验中在根管内注满无菌液体培养基,培养7d后用纸尖取样,统计检出细菌的根管个数。结果：PDT联合EDTA组杀菌率达100％,明显高于其余4组（P〈0．05）。回复实验中PDT联合EDTA组检出细菌根管数为4个,明显少于其余4组（P〈O．05）。结论：PDT联合EDTA可有效进行根管消毒,有望为临床提高根管消毒效率提供一条新途径。

Disinfection Effectiveness of Photodynamic Therapy Combined with EDTA on Infected Root Cancals in Vitro.

Objective： To investigate the disinfection effectiveness of photodynamic therapy combined with EDTA on enterococcus faecalis biofilms within infected root canals in vitro. Methods.. Seventy--five single--root premolar teeth infected with E. faecalis biofilms were divided into 5 groups. Group D served as the positive control group with teeth treated with 5.25~NaCIO. Group E served as the negative control group treated with 0.90/00 normal saline （NS）. Group A to C included teeth treated by PDT only, 17 % EDTA only, and PDT combined with EDTA（The parameters of PDT are same as Group A） . The disinfection of the E. faecalis biofilm was evaluated by colony-- forming unit（CFU） counting. After experimental treatment, each root canal was refilled with BHI for 7 days to ob- serve the growth of bacteria. Results： The sterilizing rate of PDT combined with EDTA could achieve 100~, which had a significant decrease in the number of CFUs compared with the other four groups （P〈（0.05）. The reply exper- iment showed that the number of infection root canals was only 4, which was significantly less than the other 4 groups（P（0.05）. Conclusion： PDT combined with EDTA can be effective in root caneal disinfection, which can provide an alternative method to have a thorough root canal disinfection in clinical.