自体与异体CIK细胞抗肿瘤效应的对照研究

目的比较自体细胞因子诱导的杀伤细胞(CIK)与异体CIK细胞的抗肿瘤效应。方法外周血单个核细胞诱导CIK细胞,以自体细胞单独培养为对照。用MTT法测定杀伤活性,流式细胞术分析免疫表型,ELISA法测定干扰素-γ(IFN-γ)、白介素-12(IL-12)、肿瘤坏死因子-α(TNF-α)的水平。结果异体CIK细胞增殖能力明显高于自体CIK细胞(P<0.01),CD3+CD8+、CD3+CD56+细胞比率较相同条件下自体CIK细胞组显著增多(P<0.05),培养7 d,上清液中IL-12、IFN-γ、TNF-α水平均比自体CIK细胞培养的水平高(P<0.05),对白血病细胞与淋巴瘤细胞的杀伤率显著高于自体CIK细胞(P<0.01)。结论异体CIK细胞比自体CIK细胞有更强的抗肿瘤效应。

Comparative study on anti-tumor immune response of autologous CIK cells and allogeneic CIK cells

Objective To compare the anti-tumor immune response of autologous cytokine-induced killer （CIK） cells with that of allogeneic CIK cells and explore a more effective anti-tumor adoptive immunotherapy. Methods Peripheral monocytes were isolated from patients with renal carcinoma, lung cancer, or maxillary squamous cell carcinoma and their healthy adult children. Autologous CIK cells from patients were cultured alone as controls. The killing activity was detected by MTT assay; immunophenotype changes were analyzed by flow cytometry;the IL-12 , IFN -Υ and TNF-α levels of the cultured supernatants were detected by ELISA kits. Results The results showed that the proliferation capability of allogeneic CIK cells was signifi- cantly higher than that of autologous CIK cells. Compared with autologous CIK cells , allogeneic CIK cells significantly en- hanced the anti-tumor activity and TNF-α secretion IFN-Υ secretion, reduced IL-12 secretion, increased the ratio of CD3 ＋ CD56 ＋ cells and CD3 ＋ CD8 ＋ cells. Conclusion Allogeneic CIK cells have a stronger anti-tumor effect than autologous CIK cells. The results can provide experimental evidence for clinical application of CIK cells.