射频消融猪甲状腺毁损灶形成的实验研究及意义初探

目的:探讨射频消融技术对猪甲状腺组织的影响,以了解毁损灶的形成大小与时间、功率的关系以及射频消融猪甲状腺组织的病理变化。方法:利用射频仪和外裸长5mm、直径2mm电极,分别予不同功率和时间对离体猪甲状腺组织进行消融,测量毁损灶的大小以及进行病理学检查。结果:单电极毁损灶为类圆形,在射频功率为60W时,随时间的延长,毁损坏死灶明显增加;当功率为70W时随时间的延长,毁损坏死灶范围虽增加但量有限;当功率为80W时,毁损坏死灶大小与时间没有明显相关性,大小趋于稳定。毁损区域甲状腺组织变硬,呈灰白色,与周围组织分界清楚;光学显微镜下消融区组织细胞形态和正常甲状腺组织无明显差别。结论:单根电极对甲状腺组织射频消融可形成最大直径为10.1mm的类圆形消融灶。当功率小于70W时,其射频毁损的范围大小是可以调节的,其大小与功率、时间呈正相关。

Experimental study on porcine thyroid lesion by application of radiofrequency ablation

Objective To explore the size of the porcine thyroid gland ablation lesions corresponding to the power and the time and the pathological change after radiofrequency. Methods The thyroid gland ablation lesions were made by means of radiofrequency and the monopolar electrode with the exposed tip diameter of 2 mm and tip length of 5 mm. The size of the lesion areas was measured and its pathology was studied, Results The radiofrequency lesion size was positively relevant to the power and time at the power of 60 W, and at the power higher than 70 W, the lesion size was formed to a consistent extent size and no longer enlarged. At the power of 60 W, for 30 s, 60 s and 90 s, the corresponding radiofrequency lesion size was 7.2, 8. 0 and 9.7 mm respectively. The thyroid gland became harder and off white in color after ablation. The coagulation necrosis in the lesions was showed with histological methods under light microscopy. Conclusion Application of radiofrequency ablative techniques to thyroid gland can make a lesion with diameter of 10.1mm at most. The size of the ablative lesion is fixed when the electrode is unchanged.