Nd：YAG激光照射对提高人牙周膜成维细胞增殖力的作用

目的观察Nd：YAG激光对人牙周膜成纤维细胞增殖和碱性磷酸酶（ALP）活性的影响。方法采用酶消化组织块法原代培养人牙周膜成纤维细胞，并进行波形蛋白及角蛋白的免疫细胞化学染色，对所培养的细胞进行鉴定。将牙周膜成纤维细胞分组，接受不同能量密度的Nd：YAG激光照射。测定牙周膜成纤维细胞碱性磷酸酶活性并观察其生长情况。结果5个实验组的细胞增殖速度及碱性磷酸酶含量均高于对照组，其中能量密度为60j／cm^2组、80j／cm^2组、100i／cm^2组与对照组相比有统计学的差异。结论低能量密度，短时间的Nd：YAG激光照射能促进牙周膜成纤维细胞的增殖，提高碱性磷酸酶活性。

Effects of the Nd:YAG laser radiation on the proliferation and alkaline phosphatase activity of human periodontal ligament fibroblasts

Objective To observe the effect of Nd：YAG laser on alkaline phosphatase activity and human periodontal ligament fibroblast （HPLF） proliferation HPLF proliferation and alkaline phosphatase activity after the radiation of Nd：YAG laser.） Methods HPLF were cultured after enzymatic digestion, the cells were identified by immunohistochemistry. HPLF were divided into groups and treated with Nd：YAG laser of different dose. The proliferation and alkaline phosphatase activity of different were evaluated （These groups were dynamically observed for their proliferation, and detected for their alkaline phosphatase activity）. Results The cell proliferation and ALP activity of HPLF in all the experimental groups have increased, the differences between the experimental groups which energy intensities is 60, 80,100 j/cm^2 and the control group were significant. Conclusion Low intensity of Nd：YAG laser radiation can improve the Human periodontal ligament fibroblasts proliferation and alkaline phosphatase activity.