HPLC法同时测定护肝片中五味子醇甲、五味子甲素、五味子乙素的含量

目的:对护肝片含量测定方法进行修订,并建立同时测定护肝片中五味醇甲、五味子甲素、五味子乙素含量的方法。方法:色谱柱为C18柱,流动相为甲醇-水(梯度洗脱),流量1.0 mL.min-1;检测波长220 nm。结果:五味子醇甲、五味子甲素、五味子乙素的线性范围分别为0.99～4.95μg,0.39～1.96μg,0.34～1.71μg;平均回收率分别为99.2%、96.4%、99.1%;RSD分别为1.2%、2.5%、1.5%(n=6)。结论:该方法操作简便、准确,重复性好,可用于护肝片质量控制。

HPLC Simultaneous Determination of Schisandrin, Deoxyschizandrin, SchizandrinB of Hugan Tablets

Abstract Objective： To revise the assay method of Hugan Tablets, and establish the method to determine simuhaneously schisandrin, deoxysehizandrin and schizandrin B of Hugan Tablets. Method： The mobile phase was methanol（A） -water（B） with linear gradient elution at the flow rate of 1.0 mL .min ^- 1. The detection wavelength was 220 nm. Results ： The calibration curves of schisandrin, deoxyschizandrin and schizandrin B were linear within the concentration of 0. 99-4. 95 μg, 0. 39-1.96 μgand 0. 34-1.71 μg respectively; the average recoveries were 99. 2% ,96.4% and 99. 1% respectively（ RSD = 1.2% , 2. 5% , 1.6% ,n =6）. Conclusion： The method showed a good operability, accuracy and reproducibility, can be used to the quality control of Hugan Tablets.