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| 盐炙对杜仲中京尼平苷酸体内药代动力学的影响 | |
| 作者姓名： | 高倩倩 翁泽斌 赵根华 颜翠萍 陈志鹏 蔡宝昌 徐晗 李伟东 |
| 作者单位： | 1.南京中医药大学江苏省中药炮制重点实验室，国家教育部中药炮制规范化及标准化工程研究中心，江苏 南京?210023 |
| 摘 要： | 目的?建立测定大鼠血浆中京尼平苷酸的UHPLC-MS/MS方法，研究盐炙对杜仲中京尼平苷酸药代动力学的影响。方法?色谱柱:BEH-C18（100?mm×2.1?mm，1.7?μm）；流动相:0.1%甲酸水-乙腈梯度洗脱；流速:0.3?mL/min。结果?所建立的UHPLC-MS/MS测定大鼠血浆中京尼平苷酸的方法灵敏度、选择性、稳定性较好，回收率和基质效应皆满足体内样品的测试要求。京尼平苷酸在生品和盐炙品组大鼠体内的药动学参数分别为:AUC(0-t)（1?547.18±272.28）、（2?120.694±664.532）ng·h/mL；AUC(0-∞)（1?564.42±273.97）、（2?145.61±659.983）ng·h/mL；Cmax（517.59±51.24）、(733.292±261.34)ng/mL；MRT（2.68±0.11）、（2.551±0.08）h；T1/2（1.37±0.08）、（1.43±0.17）h；Tmax（1.52±0.1）、（1.51±0.1）h。结论?杜仲盐炙后有助于促进京尼平苷酸的吸收，一定程度上增加了其生物利用度。 |
| 关 键 词： | 盐炙 杜仲 京尼平苷酸 药代动力学 |
| 收稿时间： | 5/8/2015 12:00:00 AM |
| 修稿时间： | 2015/6/17 0:00:00 |
Evaluation of Salt Processing on Pharmacokinetics of Geniposidic Acid in Eucommia ulmoides Oliver |
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| Authors: | GAO Qian-qian WENG Ze-bin ZHAO Gen-hu YAN Cui-ping CHEN Zhi-peng CAI Bao-chang XU Han LI Wei-dong |
| Institution: | 1.Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China2.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China3.Department of Clinical Medicine, Jiangsu Jumpcan Pharmaceutical Group Co., Ltd, Taizhou, 225400, China4.School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China |
| Abstract: | OBJECTIVE?To establish UHPLC-MS/MS method to determine geniposidic acid in rat plasma and to investigate the effect of salt processing on pharmacokinetics of geniposidic acid in Eucommia ulmoides Oliver. METHODS?Chromatographic column: BEH-C18（100?mm×2.1?mm， 1.7?μm); the mobile phase: acetonitrile-0.1% formic acid aqueous solution. The gradient elution program was performed at a flow rate of 0.3?mL/min. RESULTS?The main pharmacokinetic parameters of geniposidic acid in raw and salt products were that:AUC(0-t) were （1?47.18±272.28）， （2?120.694±664.532）ng·h/mL；AUC(0-∞) were （1?564.42±273.97）， （2?145.61±659.983）ng·h/mL；Cmax were （517.59±51.24）， (733.292±261.34)ng/mL；MRT were （2.68±0.11）， （2.551±0.08）h；T1/2 were （1.37±0.08）， （1.43±0.17） h；T max were （1.52±0.1）， （1.51±0.1）h. CONCLUSION?Salt processing can obviously enhance the absorption of geniposidic acid in Eucommia ulmoides Oliver， and increase the bioavailability. |
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