| 人肝脏微粒体多克隆抗体的制备 |
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| 引用本文： | 高翔,李湘晖,周尊强,李波,朱大岭,李殿俊. 人肝脏微粒体多克隆抗体的制备[J]. 哈尔滨医科大学学报, 2004, 38(5): 413-415 |
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| 作者姓名： | 高翔 李湘晖 周尊强 李波 朱大岭 李殿俊 |
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| 作者单位： | 哈尔滨医科大学,免疫学教研室,黑龙江,哈尔滨,150086;哈尔滨医科大学第二临床医学院,临床药学药物研究所,黑龙江,哈尔滨,150086;哈尔滨医科大学第一临床医学院,普外科,黑龙江,哈尔滨,150001 |
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| 摘 要： | 目的 利用肝脏微粒体制备多克隆抗体，为制备肝微粒体单克隆抗体奠定基础。方法 用肝微粒体制备抗原，免疫动物制备抗体，并用免疫沉淀、固相免疫吸附、Westem blotting法鉴定抗体特性。结果 在动物血清中检测到多克隆抗体。结论 鉴定肝微粒体多克隆抗体为将来制备肝微粒体单克隆抗体做前期工作。
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| 关 键 词： | 肝微粒体 多克隆抗体 家兔 |
| 文章编号： | 1000-1905(2004)05-0413-03 |
| 修稿时间： | 2004-02-18 |
Preparation of polyclonal antibody against human liver microsome |
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| GAO Xiang,LI Xiang-hui,ZHOU Zun-qiang,et al. Preparation of polyclonal antibody against human liver microsome[J]. Journal of Harbin Medical University, 2004, 38(5): 413-415 |
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| Authors: | GAO Xiang LI Xiang-hui ZHOU Zun-qiang et al |
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| Abstract: | Objective To prepare polyclonal antibody against liver microsome in order to prepare molecular antibody against liver microsome.Methods The antibodies were obtained by antigen immunizing animals,the antigen was prepared from liver microsome,the speciality of the antibody was identified by the methods of immunosedimentation and Western blotting.Results The polyclonal antibodies were examined in the animals' serum.Conclusion The polyclonal antibody against liver microsome which is established can be used to prepare the molecular antibody against liver microsome. |
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| Keywords: | liver microsome polyclonal antibody rabbit |
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