细胞间粘附分子－1在乙型肝炎中的表达及意义

为观察细胞间粘附分子－１（ＩＣＡＭ－１）在乙型肝炎肝组织内的表达状况，探讨ＩＣＡＭ－１在乙型肝炎肝细胞免疫损伤中的作用。采用链菌素亲生物蛋白法（ＬＳＡＢ），以ＩＣＡＭ－１的单克隆抗体，检测１０４例急、慢性乙型肝炎，９例无症状携带者，１０例正常肝组织标本内ＩＣＡＭ－１抗原表达情况。结果发现：ＩＣＡＭ－１在正常肝细胞无表达，肝窦内皮细胞弱表达；ＨＢＶ感染后肝细胞呈不同程度的表达，肝窦内皮表达增强；中、重度慢性肝炎，活动性肝硬化肝细胞ＩＣＡＭ－１表达较急性肝炎，轻度慢性肝炎显著增强（Ｐ＜０．０１）；急性肝炎较轻度慢性肝炎显著增强（Ｐ＜０．０１）；轻度慢性肝炎较无症状携带者，正常肝组织显著增强（Ｐ＜０．０１）。表明ＩＣＡＭ－１在乙型肝炎肝细胞内表达与肝细胞损伤有关，对ＨＢＶ的清除可能起着重要作用，ＩＣＡＭ－１在肝窦内皮的表达有助于淋巴细胞向肝组织内浸润。

Study on the expression and significance of ICAM-1in hepatitis B

In order to study the role of intercellular adhesion molecule-1(ICAM-1)in the mechanism of hepatocellular immune injury,the expression status of ICAM-1 was observed in hep-atitis B.Labelling streptoavidin biotin (LSAB) was a sensitive method.By using monoclonal anti-body of ICAM-1,113 liver biopsy specimens including 104 cases of acute or chronic hepatitis B and 9 cases of HBV asymptomatic carrier were examined and l0 normal liver specimens were detected in the mean time. Hepatocytes had no ICAM-1 expression andsinusoidal endotheliocytes showed low level of ICAM-1 expression in normal liver tissues.In HBV infected cases,hepatocytes demonstrat-ed ICAM-1 expression to different degrees,while sinusoidal endotheliocyte expression increased.Enhanced expression of ICAMlon hepatocyte membrane or cytoplasm was observed in moderate or severe chronic hepatitis B and in active cirrhosis in comparision with acute hepatitis B and mild chronic hepatitis B (P< 0.01).Increased expression of ICAM-1 was observed in acute hepatitis in conparision with mild chronic hepatitis(P<0.01).And expression of ICAM-1 in chronic hepatitis also increased compared to asymptomatic carrier and normal liver(P<0.01).We concluded that the expression of ICAM-1 on hepatocytes was involved in the mechanism of hepatocellular immune in-jury and might play an important role in eliminating HBV.The ICAM-1 expression of sinusoidal en-dotheliocyte contributed to lymphocyte infiltrating toward liver tissue.