姜黄素对糖尿病大鼠肾脏的保护作用及对血红素加氧酶表达的影响

目的:探讨姜黄素对糖尿病大鼠肾脏病变的作用及对肾脏血红素加氧酶-1(HO-1)表达的影响。方法:诱导大鼠糖尿病后,随机分为对照组(n=10)及治疗组(n=10),比较各组的血生化、尿微量白蛋白排泄量。采用逆转录多聚酶链反应(RT-PCR)检测HO-1 mRNA。用免疫组化检测HO-1蛋白的定位和表达水平。结果:糖尿病大鼠内生肌酐清除率(Ccr)、尿微量白蛋白(mAlb)排泄量较正常鼠增多,HO-1 mRNA和蛋白表达无明显上调。HO-1蛋白主要分布于肾小管、髓质的集合管及髓袢的上皮细胞中,肾小球表达极少。姜黄素治疗能明显降低Ccr,减少mAlb排泄量,诱导HO-1 mRNA和蛋白的表达。结论:姜黄素对糖尿病大鼠肾脏病变具有明显的防治作用,其机制可能是通过诱导HO-1的表达。

Effects of Curcumin on the Expression of Heme Oxygenase-1 in Kidney and Renal Function of Diabetic Rats

Objective: To explore the effects of Curcumin on renal lesions and its influence to expression of heme oxygenase-1(HO-1) in the kidneys of diabetic rats.Methods: Diabetic rats were randomly divided into control(n=10) and experimental group(n=10)(treated with Curcumin),blood biochemical parameters and 24 h urinary albumin excretion were compared.The level of HO-1 mRNA was examined by RT-PCR.The site and level of expression of HO-1 protein were examined by immunohistochemistry staining.Results: Compared with that in normal rats respectively,Ccr and urinary albumin excretion increased markedly in diabetic rats,but the expression of HO-1 mRNA and protein were not increased.HO-1 protein was mainly expressed in renal tubular cells,collecting ducts in medulla and Henle's loop.Ccr and urinary albumin excretion were decreased markedly and the expression of mRNA and protein of HO-1 were induced by the treatment of Curcumin.Conclusion: Curcumin has therapeutic effects on diabetic nephropathy.The mechanism is,at least in part,through induction of HO-1.