Retinal ganglion cell loss produced by intraocular kainic acid in cats: variation with somal size and eccentricity

Eight eyes of adult cats were injected with different doses of kainic acid (KA) and examined following survival times of either 5 or 12 days. At a survival time of 12 days, a dose of 76 nmol produced an 18% lossof ganglion cells in the center of the area centralis (AC), 70% loss at a location 2 mm from the AC, and 95% loss at a location 6 mm from the AC. Larger doses (240, 760 and 2400 nmol) produced losses comparable to that observed for 76 nmol. For example, 2400 nmol produced a 35% loss in the AC, 81% at 2 mm, and 88% at 6 mm. At a survival time of 5 days, doses of 240 and 760 nmol produced a loss of ganglion cells comparable to that seen at 12 days. In one eye, a large dose of KA (7600 nmol) produced total loss of ganglion cells at a survival time of 5 days. By comparing loss of cells in restricted somal diameter ranges at different retinal eccentricities, it was possible to distinguish two significant correlations that were largely independent of survival time and dose: (1) at 2 mm, loss of cells with somal diameters larger than 21 μm significantly exceeded loss of cells with smaller somata. In particular, alpha cells were totally eliminated in 6 of the 8 KA-treated eyes. (2) The mean loss of ganglion cells with somal diameters less than 21 μm was significantly greater at 2 mm and 6 mm than in the AC. Together, these results show that loss of ganglion cells produced by KA varies somal size and retinal eccentricity.