| 白细胞介素12对肝癌基因治疗的实验研究 |
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| 引用本文： | Yang J,Qian Q,Xue H,Cao H,Cui Z,Shi W,Yang G,Wu M. 白细胞介素12对肝癌基因治疗的实验研究[J]. 中华外科杂志, 1999, 37(4): 202-204 |
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| 作者姓名： | Yang J Qian Q Xue H Cao H Cui Z Shi W Yang G Wu M |
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| 作者单位： | 第二军医大学东方肝胆外科医院肿瘤免疫及基因治疗中心 |
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| 基金项目： | 国家自然科学基金,上海市现代生物与新药产业发展基金 |
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| 摘 要： | 目的 研究携带白细胞介素１２（ＩＬ－１２）基因逆转录病毒载体对体内肝癌生长抑制作用,探索基因治疗肝癌新途径。方法 构建携带ＩＬ－１２的逆转录病毒载体,转染逆转录病毒包装细胞系ＰＡ３１７,应用该包装细胞对实验性肝癌大鼠进行治疗,观察抗肿瘤作用,免疫功能变化。
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| 关 键 词： | 肝肿瘤 肝细胞癌 基因治疗 白细胞介素12 |
IL-12 gene treatment of hepatocellular carcinoma: experimental study |
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| Yang J,Qian Q,Xue H,Cao H,Cui Z,Shi W,Yang G,Wu M. IL-12 gene treatment of hepatocellular carcinoma: experimental study[J]. Chinese Journal of Surgery, 1999, 37(4): 202-204 |
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| Authors: | Yang J Qian Q Xue H Cao H Cui Z Shi W Yang G Wu M |
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| Affiliation: | Tumor Immunology and Gene Therapy Center, Eastern Institute of Hepatobiliary Surgery, Shanghai 200438. |
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| Abstract: | Objective To investigate the inhibitory effects of retrovirus vector containing IL 12 gene on hepatoma growth in vivo and to explore a new approach of gene therapy to hepatocellular carcinoma(HCC). Methods Retrovirus vector containing IL 12 gene was constructed and transfected into packaged cell PA317. Positive PA317 was injected into the rat which suffered from experimental HCC and its anti tumor effects and immunity changes were recorded. Results The packaged cell PA317 containing IL 12 gene could inhibit the proliferation of hepatocellular cell line CBRH3. The rats injected at day 1 or 3 can survive permanently, while those injected at day 5 or 7 can survive longer than those not injected. However, tumor generated in rats injected with blank control or package cells containing retroviral vector without IL 12 gene( P <0 01). Conclusions Package cells transfected with retroviral vector containing IL 12 either injected to the hepatoma tissue locally or given splenically exercises anti hepatoma effects efficiently. The direct intrasplenical injection route is new, safe and effective. |
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| Keywords: | Interleukin 12 Carcinoma hepatocellular Gene therapy |
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