铁碳复合磁性载体体内特征的研究

目的 通过吸附阿霉素的纳米铁碳复合磁性载体经猪肝动脉插管介入,观察靶区肝组织、非靶区肝组织及血清中阿霉素的药代动力学变化.方法 将香猪20头随机分为5组,分别经肝左动脉内注入阿霉素水溶液、外加磁场的高中低剂量载药铁碳复合磁性载体及不加用磁场的高剂量载药铁碳复合磁性载体,在不同时间点分别取靶区肝组织、非靶区肝组织及静脉血,质谱法测定阿霉素浓度.结果 外加磁场载药铁碳复合磁性载体组靶区肝组织内阿霉索浓度明显高于非靶区肝组织内阿霉素浓度,最高达101.4倍;也高于阿霉素水溶液组及未加用磁场组,最高达23.8倍及28.3倍.在不同剂量磁靶向治疗组中靶区肝组织内阿霉素浓度呈剂量依赖性增高,持续时间延长.结论 载药铁碳复合磁性载体在外加磁场引导下易聚集于靶区,在局部释放药物,对周围组织器官影响较小.

The characterization of iron-carbon composite magnetic carrier carrying chemotherapeutic agents in vivo

Objective To investigate the pharmacokinetics of Doxorubiein in targeted liver tissues, no-targeted liver tissues and sertrrn after Doxorubicin-adsorbed iron-carbon composite magnetic carrier was intervened through left hepatic artery in Guizhou minipigs. Methods Twenty Guizhou minipigs were divided into 5 groups, which received drugs through left hepatic artery: Doxorubicin solution group, high-,middle-,low-dose of iron-carbon composite magnetic cartier carrying Doxorubicin with outer-side magnet groups, and high dose without magnet group. The samples of targeted liver tissues, no-targeted liver tissues and serum were collected respectively to determine Doxorubicin contents by LC-MS at different time points. Results Doxorubicin content in targeted liver tissue were significantly higher than those in no- tar-geted liver tissue with the maximal level of 101.4 times. It was also 23.8 times and 28.3 times higher than that in Doxorubicin solution group and high-dose iron-carbon composite magnetic carrier without magnet group respectively. Doxorubicin content in targeted liver tissue was in a dose-and time-dependent manner with the different doses of iron-carbon composite magnetic cartier with outer-side magnet field. Conclusion Iron-carbon composite magnetic carrier carrying Doxorubicin can focus on the targeted area by magnet field ,and released the carried drug slowly in the local area with slight effect on the surrounding normal tissues.