Suitable restriction enzymes for pulsed-field gel electrophoresis analysis of Candida tropicalis |
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| Authors: | Chou Hsiao-Hui Chiou Chien-Shun Chen Kuo-Wei Li Shu-Ying |
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| Affiliation: | Mycotic Diseases Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taipei 11561, Taiwan. |
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| Abstract: | ![]()
This study was designed to pursue the identification of optimal restriction enzyme for pulsed-field gel electrophoresis (PFGE) analysis of Candida tropicalis. Twelve restriction enzymes were tested on C. tropicalis strains. Our results indicate that BssHII, NaeI, and RsrII were useful enzymes for PFGE analysis, and NaeI was the best for PFGE analysis of C. tropicalis isolates. |
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| Keywords: | Candida tropicalis PFGE Restriction enzyme Molecular typing |
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