胃癌组织中微RNA-34b/c和微RNA-124a基因的甲基化

目的 探讨DNA甲基化对微RNA(miRNA)表达的影响.方法 取2009年7至11月天津医科大学总医院新鲜活检标本胃癌组、对照组各40例.应用甲基化特异性PCR(MSP)方法检测组织中miRNA-34b/c和miRNA-124a基因启动子区甲基化.结果 miRNA-34b/c基因启动子区高甲基化阳性率在胃癌组及对照组胃黏膜中分别为77.5%(31/40)和5.0%(2/40),差异有统计学意义(P<0.05).miRNA-124a基因启动子区高甲基化阳性率在两组中分别为60.0%(24/40)和0,差异有统计学意义(P<0.05).miRNA基因启动子区高甲基化阳性率与胃癌临床病理特征有关.结论 胃癌组织存在miRNA-34b/c和miRNA-124a基因启动子区高甲基化,并可能在胃癌的发生发展中起重要作用.

Abstract：

Objective To explore the effects of DNA methylation of microRNA (miRNA) gene on their expressions in patients with gastric carcinoma.Methods A total of 80 subjects were divided into gastric carcinoma group(n=40)and control group(n=40).The DNA methylation status of miRNA-34b/c and miRNA-124a gene promoters was detected by DNA methylation specific polymerase chain reaction (MSP) in gastric carcinoma tissues and normal mucosal tissues.Results The positive rate of DNA methylation of miRNA-34b/c gene promoter was 77.5%(31/40)and 5.0%(2/40)in gastric carcinoma and control groups respectively.There was statistically significant difference between two groups (P<0.05). The positive rate of DNA methylation of miRNA-124a gene promoter was 60.0%(24/40)and 0 in these two groups respectively.There was statistically significant difference between two groups (P<0.05).Also the hypermethylation positivity of gene promoter of miRNAs was correlated with the clinicopathological features of gastric carcinoma.Conclusion The hypermethylation of miRNA-34b/c and miRNA-124a gene promoters may play a crucial role in the occurrence and development of gastric carcinoma.

DNA methylation of microRNA-34b/c and microRNA-124a in gastric cancer

Objective To explore the effects of DNA methylation of microRNA (miRNA) gene on their expressions in patients with gastric carcinoma.Methods A total of 80 subjects were divided into gastric carcinoma group(n=40)and control group(n=40).The DNA methylation status of miRNA-34b/c and miRNA-124a gene promoters was detected by DNA methylation specific polymerase chain reaction (MSP) in gastric carcinoma tissues and normal mucosal tissues.Results The positive rate of DNA methylation of miRNA-34b/c gene promoter was 77.5%(31/40)and 5.0%(2/40)in gastric carcinoma and control groups respectively.There was statistically significant difference between two groups (P<0.05). The positive rate of DNA methylation of miRNA-124a gene promoter was 60.0%(24/40)and 0 in these two groups respectively.There was statistically significant difference between two groups (P<0.05).Also the hypermethylation positivity of gene promoter of miRNAs was correlated with the clinicopathological features of gastric carcinoma.Conclusion The hypermethylation of miRNA-34b/c and miRNA-124a gene promoters may play a crucial role in the occurrence and development of gastric carcinoma.