| Comparative observation of protective effects of earplug and barrel on auditory organs of guinea pigs exposed to experimental blast underpressure |
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| 作者姓名： | 李朝军 朱佩芳 刘兆华 王正国 杨成 陈海斌 宁心 周继红 陈剑 |
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| 作者单位： | Department of Otolaryngology-Head and Neck Surgery Daping Hospital Third Military Medical University，Fourth Department of Research Institute of Field Surgery Third Military Medical University，Department of Otolaryngology-Head and Neck Surgery Daping Hospital Third Military Medical University，Fourth Department of Research Institute of Field Surgery，Department of Otolaryngology-Head and Neck Surgery，Fourth Department of Research Institute of Field Surgery，Fourth Department of Research Institute of Field Surgery，Fourth Department of Research Institute of Field Surgery，Department of Otolaryngology-Head and Neck Surgery，Chongqing 400042，China，Chongqing 400042，China，Chongqing 400042，China，Third Military Medical University，Chongqing 400042，China，Daping Hospital，Third Military Medical University，Chongqing 400042，China，Third Military Medical University，Chongqing 400042，China，Third Military Medical University，Chongqing 400042，China，Third Military Medical University，Chongqing 400042，China，Daping Hospital，Third Military Medical University，Chongqing 400042，China |
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| 摘 要： | Objective: To explore the protective effects of earplug and barrel on auditory organs of guinea pigs exposed to experimental blast underpressure (BUP). Methods : The hearing thresholds of the guinea pigs were assessed with auditory brainstem responses ( ABR). The traumatic levels of tympanic membrane and ossicular chain were observed under stereo-microscope. The rate of outer hair cells (OHCs) loss was analyzed using a light microscope. The changes of guinea pigs protected with barrel and earplug were compared with those of the control group without any protection. Results: An important ABR threshold shift of the guinea pigs without any protection was detected from 8h to 14d after being exposed to BUP with a peak ranging from -64.5 kPa to -69.3 kPa (P<0.01). The rate of perforation of tympanic membrane reached 87.5 % and that of total OHCs loss was 19.46%±5.38% at 14d after exposure. The guinea pigs protected with barrel and earplug had lower ABR threshold and total OHCs loss rate compared with the animals without any protection (P<0.01). All of the tympanic membrane and ossicular chain of the protected animals maintained their integrities. Meanwhile, the guinea pigs protected with the barrel had lower ABR threshold and total OHCs loss rate than those with earplug (P<0.01). Conclusions: The earplug and barrel have protective effects against BUP-induced trauma on auditory organs of the guinea pigs and the protective effects of barrel are better than those of earplug.
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| 关 键 词： | 保护作用 耳塞 听觉 实验研究 |
| 收稿时间： | 2005-09-09 |
Comparative observation of protective effects of earplug and barrel on auditory organs of guinea pigs exposed to experimental blast underpressure |
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| LI Chao-jun,ZHU Pei-fang,LIU Zhao-hua,WANG Zheng-guo,YANG Cheng,CHEN Hai-bin,NING Xin,ZHOU Ji-hong,Chen Jian.Comparative observation of protective effects of earplug and barrel on auditory organs of guinea pigs exposed to experimental blast underpressure[J].Chinese Journal of Traumatology(English Edition),2006,9(4):242-245. |
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| Authors: | LI Chao-jun ZHU Pei-fang LIU Zhao-hua WANG Zheng-guo YANG Cheng CHEN Hai-bin NING Xin ZHOU Ji-hong Chen Jian |
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| Institution: | 1. Department of Otolaryngology-Head and Neck Surgery,Daping Hospital, Third Military Medical University, Chongqing 400042, China
2. Fourth Department of Research Institute of Field Surgery,Third Military Medical University, Chongqing 400042, China |
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| Abstract: | OBJECTIVE: To explore the protective effects of earplug and barrel on auditory organs of guinea pigs exposed to experimental blast underpressure (BUP). METHODS: The hearing thresholds of the guinea pigs were assessed with auditory brainstem responses (ABR). The traumatic levels of tympanic membrane and ossicular chain were observed under stereo-microscope. The rate of outer hair cells (OHCs) loss was analyzed using a light microscope. The changes of guinea pigs protected with barrel and earplug were compared with those of the control group without any protection. RESULTS: An important ABR threshold shift of the guinea pigs without any protection was detected from 8h to 14d after being exposed to BUP with a peak ranging from -64.5 kPa to -69.3 kPa ( P<0.01). The rate of perforation of tympanic membrane reached 87.5% and that of total OHCs loss was 19.46% +/- 5.38% at 14d after exposure. The guinea pigs protected with barrel and earplug had lower ABR threshold and total OHCs loss rate compared with the animals without any protection (P<0.01). All of the tympanic membrane and ossicular chain of the protected animals maintained their integrities. Meanwhile, the guinea pigs protected with the barrel had lower ABR threshold and total OHCs loss rate than those with earplug (P<0.01). CONCLUSIONS: The earplug and barrel have protective effects against BUP-induced trauma on auditory organs of the guinea pigs and the protective effects of barrel are better than those of earplug. |
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| Keywords: | Guinea pigs Tympanic membrane Ear protective devices Experimental blast underpressure |
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