脂质体-c-erbB-2反义寡核苷酸对人卵巢癌裸鼠皮下移植瘤的治疗作用

目的　探讨c erbB 2反义寡脱氧核苷酸 (ASODN )对人卵巢癌裸鼠皮下移植瘤的治疗作用。方法　将 15只裸鼠建立人卵巢癌皮下移植瘤模型 ,然后随机分成 3组予以不同条件处理 :对照组 (腹腔注射转染液和脂质体 ) ,正义治疗组 (腹腔注射脂质体 c erbB 2 -SODN ) ,反义治疗组 (腹腔注射脂质体 c erbB 2 -ASODN )。治疗期间定期观测裸鼠体重和肿瘤体积 ,计算抑瘤率及肿瘤缩小率。利用RT -PCR技术比较治疗后各组肿瘤组织中c erbB 2基因水平。结果　反义治疗组与正义治疗组的抑瘤率分别为 71.2 %和 7.6% ,反义治疗组肿瘤缩小率为 2 2 .7% ,与正义治疗组相比较有显著性差异。各组裸鼠体重变化无明显差异。RT -PCR结果显示反义治疗组c erbB 2表达水平降低约 61.0 %。结论　脂质体 c erbB 2 -ASODN对人卵巢癌裸鼠皮下移植瘤有一定的治疗作用 ,可能成为日后卵巢癌基因治疗的重要途径。

The Effect of Liposome-c-erbB-2 Antisense Oligodeoxynucleotides on the Human Ovarian Carcinoma Transplanted Subcutaneously in Nude Mice

Objective To explore the effect of c erbB 2 antisense oligodeoxynucleotides(ASODN) treatment in the human ovarian epithelial cancer transplanted subcutaneously in nude mice.Methods The model of human ovarian epithelial cancer transplanted subcutaneously was established in 15 nude mice,then divided randomly into 3 groups and different treatment was given respectively(control group,sense experimental group and antisense experimental group).The weight of nude mice and tumor volume were observed,the tumor growth inhibitory and the tumor response rate was calculated,too.c erbB 2 gene expressions were compared by RT PCR after treatment among the 3 groups.Results The growth inhibitory rate in sense experimental group and antisense experimental group was 7.6% and 71.2%,respectively.The tumor response rate of antisense experimental group was 22.7%.There was no statistically difference of nude mice weight among the 3 groups.c erbB 2 expression decreased about 61.0% in the antisense experimental group.Conclusion The results suggested that there is a positive value in the human ovarian epithelial cancer transplanted subcutaneously in nude mice treated by c erbB 2 antisense oligodeoxynucleotides,it will be a more important gene therapeutic strategy for the ovarian cancer in the future.