重建手术对膝关节内侧副韧带愈合的影响

目的研究自体肌腱重建膝关节内侧副韧带后对韧带损伤愈合的影响。方法兔双侧膝关节内侧副韧带于中段完全切断做成自身对照模型。实验组模拟人半腱肌腱重建术。术后4，12，16，24周通过大体解剖和天狼猩红偏振光法观察内侧副韧带愈合的情况。结果大体解剖发现4周实验组内侧副韧带愈合组织比对照组多，术后4、12、16、24周，实验组内侧副韧带愈合组织的Ⅲ型胶原纤维与Ⅰ、Ⅲ型胶原纤维之和的平均比值分别为（0．263±0．075）、（0．235±0．041）、（0．210±0．045）、（0．197±0．029），显著低于对照组的（0．310±0．072）、（0．286±0．045）、（0．261±0．046）、（0．236±0．043）（P〈0．05）。结论半腱肌腱膝重建术可以在早、中期改善内侧副韧带的愈合。

Effect of reconstruction on medial collateral ligament healing of knee in rabbits

Objective To provide histological evidence for clinic tendon reconstruction on severe medial collateral ligament injury in rabbits. Methods Medial collateral ligaments of two knee joints were completely incised to create own control model of MCL rupture. The operation group intimated the procedure of human being MCL reconstruction with semitendinous tendon. In the 4th,12th,16th,24th weeks, healing of MCL was observed by anatomy and the picrosirius-polarization method. Results In the 4th week, the MCL healing tissue of ex-periment group is more than control group. The average ratios of type Ⅲ collagen to Ⅰ, Ⅲ collagen in the experiment group (0.263±0.075), (0.235±0.041), (0.210±0.045), (0.197±0.029)] were significantly lower than those in control group (0.310±0.072), (0.286±0.045), (0.261±0.046), (0.236±0.043) ] in the 4th, 12th, 16th, 24th weeks, respectively (P < 0.05). Conclusions The semitendinons tendon reconstruction can improve MCL healing in early and middle period after injury.