| 融合基因pCEAcd-tk/前体药物体系的体外旁观者效应的差异性 |
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| 引用本文： | 郭语彬,伍新尧,罗超权,郭俊明,许道松,刘敏.融合基因pCEAcd-tk/前体药物体系的体外旁观者效应的差异性[J].中国肿瘤生物治疗杂志,2000,7(1):42-45. |
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| 作者姓名： | 郭语彬 伍新尧 罗超权 郭俊明 许道松 刘敏 |
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| 作者单位： | 山东淄博市周村区解放军148医院!淄博255300(孙黎飞),第二军医大学免疫学教研室!上海200433(曹雪涛,田野苹,章卫平,张明徽,黄欣),第二军医大学免疫学教研室!上海20043(于益芝) |
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| 基金项目： | 国家自然科学基金!（39730420,39825123）资助 |
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| 摘 要： | 目的：观察用白细胞介素－２（ｉｎｔｅｒｌｅｕｋｉｒｔ－２,ＩＬ－２）基因修饰小鼠骨髓来源的树太细胞（ｄｅｎｄｒｉｔｉｃ ｃｅｌｌｓ,ＤＣ）后,ＤＣ表达细胞因子和趋化因子的变化及ＤＣ体内免疫后,小鼠体内主要脏器中ＩＬ－２表达情况。探讨其对抗原提呈微环境的改善及其可能的机制。方法：用ＥＬＩＳＡ方法检测ＤＣ培养上清和小鼠血清及脏器中ＩＬ－２的含量,用ＲＴ－ＰＣＲ方法检测ＤＣ中ＩＬ－２,ＩＦＮ－γ,ＩＬ－
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| 关 键 词： | 树突状细胞 基因修饰 IL－2 肿瘤 免疫疗法 |
| 收稿时间： | 1999/9/28 0:00:00 |
| 修稿时间： | 1999/12/10 0:00:00 |
Variation of Bystander Effect of E coli. CD/HSV1-tk and Prodrugs System in vitro |
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| Guo Yubin,Wu Xinyao,Luo Chaoquan,Guo Junming,Xu Daosong and Liu Min.Variation of Bystander Effect of E coli. CD/HSV1-tk and Prodrugs System in vitro[J].Chinese Journal of Cancer Biotherapy,2000,7(1):42-45. |
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| Authors: | Guo Yubin Wu Xinyao Luo Chaoquan Guo Junming Xu Daosong and Liu Min |
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| Institution: | Department of Biochemistry, Sun Yat-sen University of Medical Sciences, Guangzhou 510080;Department of Biochemistry, Sun Yat-sen University of Medical Sciences, Guangzhou 510080;Department of Biochemistry, Sun Yat-sen University of Medical Sciences, Guangzhou 510080;Department of Biochemistry, Sun Yat-sen University of Medical Sciences, Guangzhou 510080;Department of Biochemistry, Sun Yat-sen University of Medical Sciences, Guangzhou 510080;Department of Biochemistry, Sun Yat-sen University of Medical Sciences, Guangzhou 510080 |
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| Abstract: | Objective To investigate the effect of
IL-2 gene modification on expression of cytokines and chemokines in dendritic cells (DCs)
and IL-2 expression in mouse organs after in vivo DC immunization and to determine the
improvement of microenvironment of antigen presentation and its potential mechanism.
Methods The IL-2 levels in DC culture, mouse serum and organs after IL-2 gene-modified
DC(DC-IL-2) injection in vivo were examined by ELISA. The mRNA expressions of cytokine and
chemokines (IL-2, IFN-γ,
IL-12, GM-CSF, IL-4, IL-10, TNF-α, IL-1β, MIP-1β, MCP-1, RANTES and IP10) in DC-IL-2 were analyzed by
RT-PCR. The expression of IL-2Rα on surface of DC-IL-2 was analyzed by FACS. Results After
IL-2 gene modification, DCs could secret high level of IL-2, IFN-γ and IL-12. The expression of IL-2Rα
on the surface of DC-IL-2 was increased.
mRNAs of various cytokines and chemokines were detected. The levels of IL-2 increased
immediately in mouse serum, lung, spleen and liver after DC-IL-2 injection in vivo.
Conclusion The IL-2 gene modification could enhance the biologic activity of the DCs and
the microenvironment of antigen presentation was optimized. |
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| Keywords: | interleukin 2 dendritic cells gene modification cytokine chemokines |
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