| 褪黑素对人骨肉瘤细胞p53、c-Myc表达的影响 |
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| 引用本文： | 林晋,唐风华,黄铭珊,许颖,黄志鸿.褪黑素对人骨肉瘤细胞p53、c-Myc表达的影响[J].海峡药学,2020,32(6):6-9. |
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| 作者姓名： | 林晋 唐风华 黄铭珊 许颖 黄志鸿 |
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| 作者单位： | 福建卫生职业技术学院基础部,福建福州350101,福建卫生职业技术学院基础部,福建福州350101,福建卫生职业技术学院基础部,福建福州350101,福建卫生职业技术学院基础部,福建福州350101,福建医科大学公共技术中心,福建福州350122 |
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| 基金项目： | 福建省中青年教师教育科技项目资助;福建省卫生职业技术学院课题;福建卫生职业技术学院"课程思政"试点建设项目 |
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| 摘 要： | 目的研究褪黑素对人骨肉瘤MG63、U2细胞p53、c-Myc基因表达的调控。方法体外培养MG63、U2细胞,4mmol·L^-1褪黑素干预24h,实时荧光定量PCR及免疫印迹法检测p53及c-Myc基因表达情况。结果褪黑素作用后,U2细胞上调p53基因表达(P<0.05);MG63、U2细胞下调c-Myc蛋白水平表达(P<0.05)。结论褪黑素可能通过上调p53基因及下调c-Myc蛋白表达促进骨肉瘤细胞凋亡。
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| 关 键 词： | 褪黑素 骨肉瘤 P53 C-MYC 实时荧光定量PCR 免疫印迹法 |
Effects of Melatonin on the expression of p53 and c-Myc in Human Osteo-sarcoma Cells |
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| LIN Jin,TANG Feng-hua,HUANG Ming-shan,XU Ying,HUANG Zhi-hong.Effects of Melatonin on the expression of p53 and c-Myc in Human Osteo-sarcoma Cells[J].Strait Pharmaceutical Journal,2020,32(6):6-9. |
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| Authors: | LIN Jin TANG Feng-hua HUANG Ming-shan XU Ying HUANG Zhi-hong |
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| Institution: | (Department of Basic Subjects,Fujian Health College,Fuzhou 350101,China;Public technology service center,Fujian Meidcal University,Fuzhou 350122,China) |
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| Abstract: | OBJECTIVE To research the regulation of melatonin on the expression of p53 and c-Myc genes in human osteosarcoma MG63 and U2 cells.METHODS MG63 and U2 cells were cultured in vitro and treated with 4mmol·L-1 melatonin for 24h.The expression of p53 and c-Myc gene were detected by real-time fluorescence quantitative PCR and western blot.RESULTS After the treatment of melatonin the expression of p53 gene in U2 cells increased significantly(P<0.05)and the expression of c-Myc protein in MG63 and U2 cells significantly decreased(P<0.05).CONCLUSION Melatonin may promote the apoptosis of osteosarcoma cells by up-regulating the expression of p53 gene and down-regulating the expression of c-Myc protein. |
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| Keywords: | Melatonin Osteosarcoma p53 c-Myc Real-time fluorescent quantitative PCR Western blot |
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