异甘草素对创伤性脑损伤大鼠血清细胞因子的影响

目的:观察异甘草素对创伤性脑损伤大鼠血清干扰素γ(IFNγ)、单核细胞趋化蛋白-1(MCP-1)、白介素-2(IL-2)、IL-4和IL-13的影响。方法:将45只大鼠随机分为假手术组、模型组、治疗组各15只,改良Feeney法建立大鼠脑外伤模型,假手术组仅开骨窗不打击。术后治疗组给予异甘草素治疗,假手术组和模型组给予等量生理盐水,均治疗5 d。检测血清中的细胞因子,测量脑含水量,观察海马细胞形态。结果:与模型组相比,治疗组MCP-1、IL-2、IL-4和IL-13含量升高(P<0.05),IFNγ含量降低(P<0.05),脑含水量减低(P<0.05),损伤侧海马组织病理学明显改善。结论:异甘草素可促进创伤性脑损伤大鼠的恢复,其机制可能与调节细胞因子有关。

Effects of Isoliquiritigenin on Serum Cytokine levels in Rats after Traumatic Brain Injury

ObjectiveTo observe the effects of isoliquiritigenin on levels of serum IFNγ, MCP-1, IL-2, IL-4 and IL-13 in rats after traumatic brain injury （TBI）. Methods： Forty-five rats were randomly divided into sham-operated, model and treatment groups with 15 rats in each group. The TBI model was established with the modi-fied Feeney＇s method. In the rats in the sham-operated group the skull was just window-opened but without any blow. After the operation, the animals in treatment group were given isoliquiritigenin, while the rats in the sham-operated and model groups were given the same volume of normal saline. On day 5 after TBI, the serum cytokines were measured and brain water content was determined. The morphology of neurons in hippocampus was observed. Results：When compared with the model group, the level of IFNγ in the treatment group was de-creased （P〈0.05）, while the levels of MCP-1,IL-2,IL-4 and IL-13 in the treatment group were increased （P〈0.05）. The water content of brain in the treatment group was decreased compared to that in the model group（P〈0.05）. And compared with the model group, the morphology of hippocampus was improved （P〈0.05）.Conclu-sion：Isoliquiritigenin can promote brain tissue rehabilitation in rats after TBI, perhaps mediated by modulation of cytokines.