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Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells |
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| Authors: | Jin Zhou Guoping Tian Jinge Wang Xiaoguang Luo Siyang Zhang Jianping Li Li Li Bing Xu Feng Zhu Xia Wang Chunhong Jia Weijin Zhao Danyang Zhao Aihua Xu |
| Affiliation: | Jin Zhou (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Guoping Tian (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Jinge Wang (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Xiaoguang Luo (First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China)；Siyang Zhang (College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China)；Jianping Li (Liaoning Provincial Blood Center, Shenyang 110044, Liaoning Province, China)；Li Li (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Bing Xu (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Feng Zhu (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Xia Wang (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Chunhong Jia (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Weijin Zhao (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Danyang Zhao (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)；Aihua Xu (Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China)； |
| Abstract: | This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation. hUCMSCs were co-cultured with normal or Aβ1-40-injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone (no treatment). Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural cells. |
| Keywords: | stem cell umbilical cord mesenchymal stem cell co-culture induction differentiation neural cell microtubule-associated protein 2 injured cell Transwell neural regeneration regeneration |