粒细胞集落刺激因子动员内皮祖细胞治疗大鼠急性心肌梗死的疗效观察

目的 通过对心脏在体心功能和离体心肌储备功能测定，评价粒细胞集落刺激因子(G-CSF)动员内皮祖细胞(EPCs)对心肌梗死(MI)大鼠心功能改善的作用。方法 雄性Wistar大鼠36只，随机分为对照组、MI组和G-CSF组(每组12只)，MI组和G-CSF组采用冠脉左前降支结扎法制作MI模型后，分别腹腔注射生理盐水和G-CSF连续5 d。给药后第7天，球后静脉采血，测定EPCs的数目和血浆血管内皮生长因子(VEGF)及C反应蛋白(CRP)的浓度。第4周时，进行在体心功能和离体心肌储备功能的测定，对MI大鼠的心功能进行全面评价。结果 G-CSF可增加外周血中EPCs的数目，提高血浆中VEGF的浓度。同时在体心功能和离体心肌储备功能测定的结果显示，MI大鼠的心功能得到改善。结论 G-CSF能够促进心梗大鼠EPCs的动员，增强VEGF的释放，改善MI大鼠的心功能。因此，G-CSF用于EPCs动员治疗AMI在临床上是有应用前景的。

Therapeutic effects of G-CSF on rats with myocardial infarction by endothelial progenitor cell mobilization

AIM: To investigate the effects of endothelial progenitor cell (EPC) mobilization with granulocyte-colony stimulating factor (G-CSF) on neovascularization and to evaluate cardiac function. METHODS: Thirty six adult male rats were divided randomly into control group (saline), myocardial infarction group (MI) and G-CSF treated group ［G-CSF 30 g/(kg·d)］ with saline or G-CSF IP injected for 5 days. The number of EPCs was determined and serum concentrations of VEGF and CRP were measured on the seventh day. Cardiac function was evaluated in vivo and in vitro after 4 weeks. RESULTS: G-CSF mobilization increased the number of circulating EPCs. Compared to the control group, cardiac function in MI group was deteriorated, whereas that in the G-CSF treated group was significantly improved. Mobilization with G-CSF increased the serum level of VEGF and decreased CRP concentration. CONCLUSION: In rats with myocardial infarction, G-CSF promotes the mobilization of EPCs, augments the release of VEGF and improves cardiac function. EPC mobilization with G-CSF is a promising novel therapy for MI.