Biocompatibility of PCL/PLGA-BCP porous scaffold for bone tissue engineering applications |
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| Authors: | Nguyen Thi Hiep Huynh Chan Khon Nguyen Dai Hai Lee Byong-Taek Vo Van Toi Le Thanh Hung |
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| Affiliation: | 1. Tissue Engineering and Regenerative Medicine Laboratory, Biomedical Engineering Department, International University, Vietnam National University-Ho Chi Minh City (VNU-HCMC), Ho Chi Minh City, Vietnam;2. Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam;3. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam;4. Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, South Korea;5. Faculty of Odonto-Stomatology, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam |
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| Abstract: | ![]()
In this study, biomimic porous polycaprolactone/poly (lactide-co-glycolide) loading biphasic tricalcium phosphate (PCL/PLGA-BCP) scaffolds were fabricated successfully by solvent evaporation method. The distribution of biphasic tricalcium phosphate (BCP) in polycaprolactone/poly (lactide-co-glycolide) (PCL/PLGA) scaffold was confirmed by micro-computed tomography (micro-CT) scanning, scanning electron microscope (SEM) observation and Energy-dispersive X-ray Spectroscopy (EDS) analysis. The hydrophilicity of the scaffolds was confirmed by contact angle measurement. In in vitro experiments, proliferation of human bone marrow mesenchymal stem cell (hBMSCs) and its osteoblastic differentiation on scaffold were assessed for 1, 2 and 3 weeks using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, fluorescence observation, hematoxylin & eosin (H&E) staining and real-time polymerase chain reaction (RT-PCR). In in vivo experiments, ossification was observed using micro-CT analysis and histological staining. |
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| Keywords: | Poly (lactide-co-glycolide) polycaprolactone biphasic calcium phosphate bone regeneration |
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