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| 中药银杏叶提取物联合腺病毒介导神经营养素-3基因的神经干细胞移植治疗急性脊髓损伤的实验研究 | |
| 引用本文： | 张文贤,吕江宏,白登彦,李盛华,冯康虎,徐玉德. 中药银杏叶提取物联合腺病毒介导神经营养素-3基因的神经干细胞移植治疗急性脊髓损伤的实验研究[J]. 中国中医骨伤科杂志, 2010, 0(5): 16-18 |
| 作者姓名： | 张文贤 吕江宏 白登彦 李盛华 冯康虎 徐玉德 |
| 作者单位： | 天津中医药大学;甘肃省中医院;甘肃省第二人民医院骨科; |
| 摘 要： | 目的:明确经腹腔注射中药银杏叶提取物是否对腺病毒介导NT-3基因的神经干细胞移植治疗急性脊髓损伤存在干预作用。方法:采用ALLEN打击法造模成功后随机分组:即模型对照组(A)、腺病毒介导NT-3基因的神经干细胞移植组(B)、中药银杏叶提取物联合腺病毒介导NT-3基因的神经干细胞移植组(C)、单纯腹腔注射中药银杏叶提取物组(D)。于2、6、8周各组大鼠取材,进行运动功能评分、每组5只行电生理检查、免疫组织化学染色、图像分析。结果:C组8周后大鼠BBB评分为6.24±0.15;术后2周,B组和C组的皮层体感诱发电位消失,移植后8周B、C组的波形恢复,但潜伏期延长(P0.05)。C组与B组比较潜伏期明显缩短(P0.05)。术后8周C组阳性细胞主要见于脊髓室管膜区,与B组比较染色面积较大,着色较深;术后2周,各组动物皮质脊髓束均有液化灶形成,6周后B、C组皮质脊髓束损伤区逐渐有新生的神经纤维束穿过。8周后B、C组皮质脊髓束损伤区新生的神经纤维束明显增多(P0.05)。结论:中药银杏提取物联合腺病毒介导NT-3基因的神经干细胞移植能够促进损伤神经元的修复或再生,明显改善损伤脊髓的传导功能,增加皮质脊髓束损伤区的神经纤维束数目,对急性脊髓损有临床治疗作用。 |
| 关 键 词： | 中药银杏叶提取物 腺病毒 神经营养素-3 神经干细胞 急性脊髓损伤 |
Ginkgo Biloba Leaf Extract combined with Transplantation of Nerve Stem Cells Transfected with Neurotrophin-3 in Treating Acute Spinal Cord Injury |
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| ZHANG Wenxian LV Jianghong BAI Dengyan LI Shenghua FENG Kanghu XU YudeTianjin University of Traditional Chinese MedicineGansu Hospital of Traditional Chinese Medicine. Ginkgo Biloba Leaf Extract combined with Transplantation of Nerve Stem Cells Transfected with Neurotrophin-3 in Treating Acute Spinal Cord Injury[J]. Chinese Journal of Traditional Medical Traumatology & Orthopeics, 2010, 0(5): 16-18 | |
| Authors: | ZHANG Wenxian LV Jianghong BAI Dengyan LI Shenghua FENG Kanghu XU YudeTianjin University of Traditional Chinese MedicineGansu Hospital of Traditional Chinese Medicine |
| Affiliation: | ZHANG Wenxian1 LV Jianghong1 BAI Dengyan2 LI Shenghua1 FENG Kanghu1 XU Yude11Tianjin University of Traditional Chinese Medicine2Gansu Hospital of Traditional Chinese Medicine3Department of Orthopedics,the 2nd People's Hospital of Gansu,Lanzhou 730000,China |
| Abstract: | Objective:To observe the effect of ginkgo biloba leaf extract combined with transplantation of nerve stem cells transfected with neurotrophin-3(NT-3)for acute spinal cord injury(ASCI).Methods:After the animal models of ASCI were established by Allen's method,they were randomized into model control(A),transplantation of nerve stem cells transfected with NT-3 mediated by adenovirus group(B),nerve stem cell transplantation plus ginkgo biloba leaf extract group(C),and ginkgo biloba leaf extract group(D).At the ... |
| Keywords: | Extract of Ginkgo Biloba Leaf Adenovirus Neurenergen-3 Nerve Stem Cell Acute Spinal Cord Injury |
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