视神经切断再生实验研究

目的:观察家兔视神经切断后再生改变。方法:采用硫喷妥钠腹腔内注射联合球后20g/L奴夫卡因溶液注射麻醉,剪开球结膜用视神经钳分离视神经,用显微手术剪将视神经2/3切断,30d后手术取出视神经,进行病理染色检查。结果:实验30眼有28眼再生,视神经切断处间隙被再生的视神经纤维长满接通,再生的视神经纤维比正常的粗大,排列不整齐,呈条索状。结论:视神经损伤是可再生的,其再生形式从切口两侧缘开始,而不像周围神经那样从近端开始向远端再生。

Experimental study on the regeneration of optic nerve after neurotomy in rabbit

AIM: To observe regeneration of rabbit optic nerve after axotomy.·METHODS: Optic nerve was separated by clipper after injecting sodium pentothal intra-peritoneaiiy associating with retrobuibar injection of 20g/L novocaine. 2/3 optic nerve was excised by microsurgical scissors. On postoperative 30 days, optic nerve was taken and undergone pathological staining test. ·RESULTS: Regeneration happened in 28 eyes of all the 30 eyes. Nerve fibers overgrew in the blank of excised optic nerve. Regenerated nerve fibers were more coarse and disordered than normal nerve fibers. ·CONCLUSION: Impaired optic nerve can regenerate. It happens from two sides of incision, instead of growth from proximate to disal end like peripheral nerve.