雌激素对急性心肌梗死前后去卵巢大鼠干细胞及心功能的影响

目的去卵巢大鼠发生急性心肌梗死前后,应用雌激素干预,观察其外周血干细胞的变化及其对心功能的影响。方法将30只SD雌性大鼠分为正常对照组、急性心肌梗死组、去卵巢心肌梗死组、去卵巢雌激素替代组及去卵巢雌激素治疗组。用流式细胞术检测各组CD90 细胞的百分率;用POWERLAB4.12系统测量左室收缩末压(LVESP)、左室舒张末压(LVEDP)、 dp/dtmax及-dp/dtmax。结果去卵巢心肌梗死组外周血CD90 细胞的百分率显著低于急性心肌塞组及去卵巢雌激素替代组(P<0.01);而去卵巢雌激素替代组心梗后第1天外周血CD90 细胞的百分率即显著升高(P<0.05),第3天达峰值,且数值较高。去卵巢雌激素治疗组与去卵巢心肌梗死组相比较,仅在心梗后7d外周血CD90 细胞的百分率才开始升高;但与去卵巢雌激素替代组相比,心梗术后7d内各时点均较低。各组LVESP均有所下降,以急性心肌梗死组为著;而LVEDP均有升高,以去卵巢心肌梗死组及去卵巢雌激素治疗组最明显(P<0.01)。结论去卵巢大鼠在心肌梗死之前应用雌激素替代治疗,外周血CD90 细胞的百分率较高,且心功能明显优于去卵巢大鼠在心肌梗死后应用雌激素替代治疗。

Effects of estradiol intervention on peripheral blood CD90 + cells and cardiac function in de-ovary rats before and after myocardial infarction

AIM: To investigate the effects of estradiol intervention on peripheral blood CD90 cells and cardiac function in de-ovary rats before and after myocardial infarction. METHODS: Thirty SD female rats were divided into the control group, acute myocardial infarction group, de-ovary myocardial infarction group, de-ovary replacement group with estradiol, and de-ovary therapy group with estradiol. CD90 cell percentage was determined with flow cytometry. The left ventricle end systolic pressure (LVESP), left ventricle end diastolic pressure (LVEDP), dp/dt_ max and-dp/dt_ max were also measured with the POWERLAB4.12 system. RESULTS: The percentage of peripheral blood CD90 cells in the de-ovary myocardial infarction group was remarkably lower than that in the acute myocardial infarction group and the de-ovary replacement group with estradiol (P<0.01) . In the de-ovary replacement group with estradiol, the percentage of peripheral blood CD90 cells remarkably elevated following the first day of operation (P<0.05), reaching the peak value at day 3. In the de-ovary therapy group with estradiol, it started to elevate at day 7 after the operation, much lower than that in the de-ovary replacement group with estradiol. LVESP in each operation group decreased, especially in the acute myocardial infarction group, whereas LVEDP increased in the de-ovary group and de-ovary therapy group with estradiol (P<0.01). CONCLUSION: Replacement with estradiol before myocardial infarction exerts more beneficial effects than estradiol replacement after myocardial infarction in de-ovary rats.