Adsorption of Human Recombinant Erythropoietin on Dialysis Membranes In Vitro |
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| Authors: | Hidetaro Mori Keisuke Hiraoka Ryoichi Yorifuji Tohru Iwasaki Syuzo Gomikawa Ohshi Inagaki Seishi Inoue Yoshihiro Takamitsu Yoshikazu Fujita |
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| Affiliation: | Renal and Dialysis Unit, Hyogo College of Medicine, Nishinomiya City, Hyogo Prefecture, Japan |
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| Abstract: | Abstract: The adsorptive characteristics of 5 dialysis membranes for recombinant human erythropoietin (EPO) were studied in vitro in a closed circuit system. For 120 min, EPO added with bovine serum was significantly adsorbed by polymethylmetacrylate (PMMA) and polyacry–lonitrile (PAN) membranes but not by Cuprophan, ethylene vinyl alcohol (EVAL), or polysulfone (PS) membranes. In addition the EPO adsorptive rate, as well as that of β2–microglobulin (β2–MG), was greater with a PMMA membrane than with a PAN membrane. EPO was not detected in the ultrafiltrate at 15 min with 5 membranes. These results indicate that EPO was eliminated by membrane adsorption only with some dialysis membranes. |
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| Keywords: | Highly permeable membrane Adsorption Recombinant human erythropoietin β2–microglobulin |
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