| 诱导型一氧化氮合酶基因在3T3成纤维细胞中的转染和意义 |
| |
| 引用本文： | 张春雷,蒲晓允,邓均,李招权,黄辉. 诱导型一氧化氮合酶基因在3T3成纤维细胞中的转染和意义[J]. 重庆医学, 2004, 33(6): 873-875 |
| |
| 作者姓名： | 张春雷 蒲晓允 邓均 李招权 黄辉 |
| |
| 作者单位： | 第三军医大学临床检验系血液学教研室,重庆,400038;第三军医大学临床检验系血液学教研室,重庆,400038;第三军医大学临床检验系血液学教研室,重庆,400038;第三军医大学临床检验系血液学教研室,重庆,400038;第三军医大学临床检验系血液学教研室,重庆,400038 |
| |
| 摘 要： | 目的观察人诱导型一氧化氮合酶基因(iNOS)转染3T3成纤维细胞的可能性.方法用阳性脂质体将含人诱导型一氧化氮的真核表达载体pcDNA3.0-iNOS转染至3T3细胞,用G418筛选,通过RT-PCR、免疫组化的方法鉴定.结果 pcDNA3.0-iNOS基因转染的3T3细胞有人iNOS基因mRNA的表达,细胞中有iNOS蛋白的表达,空载体和对照组没有表达.结论人iNOS基因能够在3T3成纤维细胞得到稳定转染和表达.
|
| 关 键 词： | 一氧化氮 基因转染 诱导型一氧化氮合酶 |
| 文章编号： | 1671-8348(2004)06-0873-03 |
Expression and significance of human inducible nitric oxide synthase gene in 3T3 cells |
| |
| ZHANG Chun lei,PU Xiao yun,DENG Jun,et al.. Expression and significance of human inducible nitric oxide synthase gene in 3T3 cells[J]. Chongqing Medical Journal, 2004, 33(6): 873-875 |
| |
| Authors: | ZHANG Chun lei PU Xiao yun DENG Jun et al. |
| |
| Abstract: | Objective To observe the feasibility of transfection human nitric oxide synthase in 3T3 cells.Methods The eukaryotic expression vector pcDNA3.0 iNOS was transfected into 3T3 cells.After the screening with G418 and cloning,the cells were verified with RT PCR and immunofluorescence staining.Results The expression of iNOS mRNA and iNOS protein in 3T3 cells transfected with pcDNA3.0 iNOS was detected.There is no the same expression in 3T3 cells and transfected with pcDNA3.0 3T3 cells.Conclusion The 3T3 cells can be transfected successfully. |
| |
| Keywords: | nitric oxide gene transfer inducible nitric oxide synthase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
