| 红景天苷对新生大鼠海马区神经干细胞分化的研究 |
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| 引用本文： | 张维烨,程桂芹,宋军华,杨宇,韩磊,李艳君. 红景天苷对新生大鼠海马区神经干细胞分化的研究[J]. 解剖科学进展, 2008, 14(2): 205-208 |
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| 作者姓名： | 张维烨 程桂芹 宋军华 杨宇 韩磊 李艳君 |
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| 作者单位： | 山东省青岛卫校,青岛,266071;佳木斯大学基础医学院,佳木斯,154007 |
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| 摘 要： | 目的研究红景天苷药物血清诱导新生大鼠海马神经干细胞向神经元方向分化,并促进所分化神经元细胞发育的作用。方法从新生24h内的Wistar大鼠脑中分离扩增获得大量神经干细胞后,加入低、中、高剂量的红景天苷药物血清及对照血清,观察其对神经干细胞分化为神经元的影响,并通过免疫细胞化学染色检测神经干细胞分化为神经元的状况。结果各药物实验组诱导神经干细胞分化为NSE阳性细胞的个数面积与周长明显高于对照组(P<0.05),分化为GFAP阳性细胞的个数明显低于对照组,且呈量效依赖关系。结论红景天苷药物学清在体外可促进神经干细胞向神经元方向分化,在一定范围内存在量效依赖关系,对所分化的神经元细胞有促生长发育作用。
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| 关 键 词： | 神经干细胞 红景天苷 诱导分化 发育 |
| 文章编号： | 1006-2947(2008)02-0205-04 |
| 修稿时间： | 2007-07-25 |
The Effect of Salidroside on the Differentiation of Neural Stem Cell into Nerve Cells in Hippocampi of Newborn Rats |
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| ZHANG Wei-ye,CHENG Gui-qin,SONG Jun-hua,YANG Yu,HAN Lei,LI Yan-jun. The Effect of Salidroside on the Differentiation of Neural Stem Cell into Nerve Cells in Hippocampi of Newborn Rats[J]. Progress of Anatomical Sciences, 2008, 14(2): 205-208 |
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| Authors: | ZHANG Wei-ye CHENG Gui-qin SONG Jun-hua YANG Yu HAN Lei LI Yan-jun |
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| Abstract: | Objective To study the effect of Salidroside on the differentiation of neural stem cells into nerve cells in hippocampi of newborn rat.Methods Neural stem ceils from newborn(within 24 hours)Wistar rat brain were isolated and amplified and observed the effect of Salidroside serum on the differentiation by immunocytochemistry staining.Results Compared with control,the number of neuron specific enolase(NSE )positive cells and glial fibrillary acidic protein (GFAP) positive cells in experimental group were increased(P<0.05)and decreased(P<0.05)respectively,the area and perimeter of NSE cells were obviously larger(P<0.05)with positive dose dependent.Conclusion Salidroside serum promotes the differentiation of neural stem cell into nerve cell and boost the growth of nerve cell. |
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| Keywords: | neural stem cell Salidroside induced differentiation growth |
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