HPLC法测定丹枣口服液中丹参素及原儿茶醛的含量

采用HPLC法同时测定丹枣口服液中的丹参素及原儿茶醛。方法：选用Kromasil ODS C18色谱柱（200mm×4．6mm，5μm），流动相甲醇-1％冰醋酸（12：88），检测波长280nm，流速1．0ml·min^-1。结果：丹参素钠、原儿茶醛分别在进样量0．28～2．82μg（r=0．999 5），0．05～0．55μg（r=0．999 9）范围内呈良好线性关系。丹参素钠、原儿茶醛平均回收率分别为100．9％（RSD=2．5％），99．3％（RSD=2．2％，n=9）。结论：方法简便、准确，重复性好，可用于该制剂的质量控制。

Determination the Contents of Sodium Danshensu and Protocatechuic Aldehyde in Danzao Oral Liguid by HPLC

To established a HPLC method for determination the contents of sodium Danshensu and protocatechuic aldehyde in Danzao oral liquid. Method ： A Kromasil ODS C18 column （ 200 mm × 4.6 mm, 5 μm） was used, the methanol- 1% glacial acetic acid solution（ 12： 88） as mobile phase, the flow rate was 1.0 ml·min^-1, the detection wavelength was 280 nm. Result： The linear ranges of sodium Danshensu and protocatechuic aldehyde were at 0.28 - 2.82μg （ r = 0. 999 5 ） and 0.05 - 0.55 μg （ r = 0. 999 9 ） , the average recoveries （ n = 9） were 100.9 % and 99.3 % with RSD 2.5 % and 2.2%, respectively. Conclusion： The method is simple, accurate and repeatability, it can be used for quality control of Danzao Oral liquid.