注射糖皮质激素和慢性束缚应激抑郁样大鼠模型的比较及其机制探讨

目的 比较束缚应激和注射糖皮质激素两种应激抑郁造模方法以及神经内分泌机制的探讨.方法 24只雄性Sprague Dawley(SD)大鼠随机分为正常对照组(NC组,8只), 束缚应激组(MS组,8只)和糖皮质激素组(CO组,8只),用长期束缚制动方法和注射氢化可的松建立大鼠应激抑郁模型,建模时间21天,每周进行体质量、1%蔗糖水消耗、旷场试验测定,造模结束后用逆转录-聚合酶链反应(RT-PCR)检测各组大鼠海马区糖皮质激素受体(Glucocorticoid receptor ,GR)mRNA表达的变化.结果 ① MS组和CO组大鼠体质量增加量,1%蔗糖水消耗量,行为学水平评分和垂直评分在第3周末均较NC组明显降低(F=26.36, 16.66, 18.72,13.36,P<0.01).②MS组和CO组在第2周和第3周各项行为学评分差异无统计学意义.③建模后MS组和CO组GRmRNA表达较NC组明显降低(F=30.126,P<0.01).结论 两种造模方法均能较好模拟了抑郁症的精神运动迟滞和快感缺失,是可靠的应激抑郁动物模型,而GRmRNA表达的变化是应激介导抑郁样反应的神经内分泌机制之一.

Chronic restraint stress and glucocorticoid stress in an experimental model of depression in rat

Objective:To study the validity,reliability of restraint stress and glucocorticoid stress depression models and explore the neuroendocrine mechanism of depression.Methods:Designated 24 SD male rat stochastically divides into the normal control groups(NC,n=8),the mental stress groups(MS,n=8),the glucocorticoid groups(CO,n=8),MS groups received 21 days of chronic restraint stress,and CO groups were injected glucocorticoid 20mg/kg.d for same days.The behavior of rats was tested at each weekend.Using reverse tr...