首页 | 本学科首页   官方微博 | 高级检索  
     

救心复脉注射液对豚鼠心室肌细胞L型钙电流的影响
引用本文:方芳,宋涛,谢立新,梁宋平,刘美丽,周宏灏,方云祥. 救心复脉注射液对豚鼠心室肌细胞L型钙电流的影响[J]. 中南大学学报(医学版), 2004, 29(5): 521-524
作者姓名:方芳  宋涛  谢立新  梁宋平  刘美丽  周宏灏  方云祥
作者单位:中南大学药学院,长沙410078;湖南师范大学生命科学院,长沙,410081;天津医药工业研究所,天津,300193;中南大学临床药理研究所,长沙,410078
基金项目:国家自然科学基金 (85 91 9 0 30 2 )
摘    要:
目的:探讨救心复脉注射液(枳实提取液)有效成分辛弗林(311)、N-甲基酪胺(417)对豚鼠心室肌细胞膜L型钙通道电流(ICa-L)的影响。方法:用酶解法分离单个心室肌细胞,采用全细胞膜片钳技术,观察辛弗林、N-甲基酪胺对豚鼠心室肌细胞膜ICa-L的影响。结果:用辛弗林(10,25,50,100 mmol/L)时能增大 ICa-L ,增加率分别为 8.27%,27.29%,41.01% 和 48.74% (P<0.05)。 用N-甲基酪胺(10,25,50,100 mmol/L) 时能增大 ICa-L ,增加率分别为 10.05%, 30.12%,43.05% 和51.90%(P<0.05)。辛弗林和N-甲基酪胺只改变电流幅度,不改变I-V曲线形状。结论:辛弗林和N-甲基酪胺有浓度依赖性的增大豚鼠心室肌细胞ICa-L,促进钙通道开放的作用。

关 键 词:中药  心室肌细胞  钙电流  膜片钳  豚鼠
文章编号:1672-7347(2004)05-0521-04
修稿时间:2003-09-29

Effect of Jiuxinfumai injection on L-type calcium currents in single guinea pig ventricular myocytes
FANG Fang ,SONG Tao ,XIE Li-xin ,LIANG Song-ping ,LIU Mei-li ,ZHOU Hong-hao ,FANG Yun-xiang . Effect of Jiuxinfumai injection on L-type calcium currents in single guinea pig ventricular myocytes[J]. Journal of Central South University. Medical sciences, 2004, 29(5): 521-524
Authors:FANG Fang   SONG Tao   XIE Li-xin   LIANG Song-ping   LIU Mei-li   ZHOU Hong-hao   FANG Yun-xiang
Affiliation:1.School of Pharmaceutical Sciences, Central South University, Changsha, 410078; 2.School of Life Sciences,
Hunan Normal University,Changsha 410081;  3.Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China;
4.Institute of Clinical Phamocology,Central South University,Changsha 410078,China
Abstract:
Objective To determine the effect of 311 and 417, both active ingredients isolated from Jiuxinfumai injection (Citrus Aurantium) on L-type calcium currents (I Ca-L)in ventricular myocytes of guinea pigs.Methods Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of 311and 417. Results 311(10,25,50,100 mmol/L)increased the I Ca-L by 8.27%, 27.29%, 41.01%,and 48.74% (P<0.05), respectively. 417(10,25,50,100 mmol/L) increased the I Ca-L by 10.05%, 30.12%,43.05%,and 51.90% (P<0.05), respectively. Both 311 and 417 changed the I Ca-L significantly in a concentration-dependent manner. They did not change the shape of I-V cruves.Conclusion 311 and 417 can increase I Ca-L in ventricular myocytes of guinea pigs in a dose-response manner.
Keywords:Chinese materia medica  ventricular myocyte  L-type calcium current  patch clamp  guinea pigs
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中南大学学报(医学版)》浏览原始摘要信息
点击此处可从《中南大学学报(医学版)》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号