束缚应激上调小鼠脾脏淋巴细胞钾离子通道(Kv1.3)的表达

目的观察束缚应激条件下小鼠脾脏细胞Kv1.3表达的变化。方法采用RT-PCR、Western blot等分子生物学及免疫组化实验方法,分别从基因和蛋白质水平揭示束缚应激对小鼠脾脏细胞Kv1.3表达的调节。结果束缚应激16 h后小鼠脾脏细胞Kv1.3 mRNA和蛋白质表达水平明显上调(P<0.05)。但是,相同束缚应激条件下小鼠脑组织Kv1.3表达无明显变化(P>0.05)。结论束缚应激状态下小鼠脾脏细胞Kv1.3 mR-NA和蛋白质表达水平呈明显组织特异性上调,提示束缚应激条件下免疫功能的调节可能与钾通道有关。这为进一步研究神经内分泌系统调节淋巴细胞功能的机制提供了新的思路。

Up-regulation of Kv1.3 Expression in Mice Splenocytes Induced by Restraint Stress

Objective To investigate the variety of voltage-gated potassium channel(Kv1.3) expression in splenocytes during restraint stress.Methods RT-PCR,Western blot and immunohistochemical staining were performed to demonstrate the mRNA and protein expression level of the Kv1.3 in mouse splenocytes.Results Kv1.3 mRNA and protein expression were significantly up-regulated in the splenocytes of restraint stressed mice compared with that of control mice.However,immunohistochemical staining results indicated that there was no statistical different of the Kv1.3 expression in the brain between normal and restraint stressed mice.Conclusions Kv1.3 mRNA and protein expression was organ-specific up-regulating splenocytes of mice after restraint stress.It suggests that potassium channels(Kv1.3) may play an important role in regulation of the immune function during restraint stress.Thus,it may provide us a new sight to understand the mechanism of stress regulating the lymphocyte activation by potassium channels.