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| p16基因甲基化对胃癌的早期诊断 | |
| 引用本文： | 李士军,袁宏,刘新元. p16基因甲基化对胃癌的早期诊断[J]. 中国误诊学杂志, 2005, 5(4): 607-609 |
| 作者姓名： | 李士军 袁宏 刘新元 |
| 作者单位： | 大连医科大学附属第一医院检验科,辽宁,大连,116011 |
| 摘 要： | 目的:检测胃癌患者癌组织及全血DNAp16基因甲基化,并探讨其在胃癌早期诊断中的作用。方法:收集新鲜的胃癌组织及血液标本4 4例,胃溃疡等胃部良性疾病患者标本2 0例。采用甲基特异性PCR(MSP)技术分别检测肿瘤组织DNA和全血DNA中p16基因启动子甲基化。结果:38.6 % (17/ 4 4 )胃癌组织出现p16基因甲基化,出现甲基化组织对应的血液标本中88.2 % (15 / 17)出现p16基因甲基化。胃溃疡等胃部良性疾病患者标本中未发现甲基化。肿瘤组织中p16基因的甲基化状况与全血中出现p16基因甲基化关系密切(P<0 .0 1)。结论:胃癌组织及血液标本中p16基因甲基化的检测有助于胃癌的早期诊断。 |
| 关 键 词： | 胃肿瘤／诊断 蛋白质p16／代谢 蛋白质p16／遗传学 甲基化 基因，p16 |
| 文章编号： | 1009-6647(2005)04-0607-03 |
| 修稿时间： | 2004-12-25 |
The Clinical Study of the Methylation in Gene P16 in Early Diagnosis in Stomach Cancer |
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| LI Shi-jun,YUAN Hong,LIU Xin-yuan. The Clinical Study of the Methylation in Gene P16 in Early Diagnosis in Stomach Cancer[J]. Chinese Journal of Misdiagnostics, 2005, 5(4): 607-609 | |
| Authors: | LI Shi-jun YUAN Hong LIU Xin-yuan |
| Abstract: | Objective:To detect methylation in p16 gene in whole blood and tissue of stomach cancer, and to explore its significance for early diagnosis in stomach cancer.Method:44 tissue samples and their corresponding whole blood samples from patients with stomach cancer, 20 tissue samples with gastric ulcer were collected for methylation measurement by PCR technique.Result:Methylated p16 gene was found in 38.6%(17/44)of stomach cancer and in (88.2%)(15/17) whole blood samples, no methylated p16 gene was found in gastric ulcer. Methylated p16 gene promoter in whole blood strongly correlated with tissue of stomach cancer.Conclusion:The measurement of methylated p16 gene should be helpful to early diagnosis in stomach cancer. |
| Keywords: | Stomach neoplasms/diagnosis Protein p16/metabolism Protein p16/genetics Methylation Geges p16 |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |