稀土化合物氯化镧对脂多糖诱导小鼠巨噬细胞分泌-一氧化氮的影响

目的探讨氯化镧（LaCl3）由对脂多糖（Lipopolysaccharide，LPS）刺激小鼠巨噬细胞（RAW264．7）分泌一氧化氮（nitric oxide，N01的影响。方法RAW 264．7细胞随机分成四组：空白对照组（培养基中不含LaCl3和LPS），氯化镧组（含LaCk2．5，5，10，20μmol／L培养24h）、氯化镧＋LPS组（分别以含LaCl3 2．5、5、10、20μmol／L的培养基培养24h后．更换含LPS 1mg／L的培养基继续培养24h）及LPS组（含LPS 1mg／L的培养基培养24h）。采用硝酸还原酶法测定各组细胞培养液上清一氧化氮（NO）的含量。结果LPS组培养上清NO浓度为52．82±12．60μmol／L与空白对照组（2．90±2．36μmol／L）和LaCl3组（6．50±4．67μmol／L、9．52±4．47μmol／L、11．14±7．42μmol／L、6．74±2．00μmol／L]比较有显著性差异（P〈0．05）；LaCl3＋LPS组NO的浓度分别为26．00±5．83μmol/L（2．5μmol／L LaCl3＋LPS）、29．86±7．26μmol／L（5μmol／L LaCl3＋LPS）、34．62±6．24μmol／L （10μmol／L LaCl3＋LPS），与LPS组相比显著减少（P〈0．05），20μmol／L LaCl3＋LPS）组NO的产生为45．61±6．68μmol／L与LPS组比较无显著性差异。结论一定浓度的LaCl3可抑制LPS诱导小鼠巨噬细胞产生NO的水平。

Effects of Lanthanum chloride on the production of nitric oxide in RAW 264.7 macrophages induced by lipopolysaccharide

Objective To explore the effects of lanthanum chloride（LaCl3） on nitric oxide（NO） production in RAW 264.7 maerophages with Lipopolysaeeharide （LPS） induction. Methods The cells were randomly divided into four groups： control group （RAW 264.7 cells were incubated without LaCl3 or LPS）, LaCl3 group （cells cultured in nedium eontatining 2.5, 5, 10, 20μmol/L of LaCl3）, LaCl3＋LPA group （after incubated with 2.5, 5, 10 20μmol/L of LaCl3 FOR 24h, cells were stimulated with lmg/L of LPS）, and LPS group （cells cultured in medium eontatining lmg/L of LPS）, then the cells were harvested after 24h. NO production in culture supernatant was assayed by measuring nitrite. Results NO production assay indicated that the concentration of NO in culture supernatant of LPS group was 52.82±12.60μmol/L, significantly higher than that of control group （2.90±2.36μmol/L） and LaCl3 group （6.50±4.67μmol/L, 9.52±4.47μmol/L, 11.14±7.42μmol/L, 6.74±2.00μmol/L） （P〈0.05）; Whereas in the LaCl3＋LPS group, the concentration of NO was 26.00±5.83μmol/L（2.5μmol/L LaCl3＋LPS）, 29.86±7.26μmol/L （5μmol/L LaCl3＋LPS）, 34.62±6.24μmol/L （10μmol/L LaCl3＋LPS） and 45.61±6.68μmol/L （20μmol/L LaCl3＋LPS）, the first three of which decreased significantly compared with LPS group （P〈0.05）. Conclusion LaCl3 reduces production of NO in RAW 264.7 cells induced by LPS.