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| 结核分枝杆菌耐利福平rpoB基因突变的序列分析 | |
| 引用本文： | 甘辛,陈玲,王建华,李娜娜,陈杨,张建勇,张泓. 结核分枝杆菌耐利福平rpoB基因突变的序列分析[J]. 基础医学与临床, 2010, 30(3): 268-271 |
| 作者姓名： | 甘辛 陈玲 王建华 李娜娜 陈杨 张建勇 张泓 |
| 作者单位： | 遵义医学院,附属医院,呼吸内科,贵州,遵义,563003 |
| 基金项目： | 贵州省科学技术基金(黔科合J字[2007]2223号);;美国疾病控制与预防中心(美国CDC)资助项目(CDCSBIR Award#200-2007-M-22792) |
| 摘 要： | 目的探讨结核分枝杆菌耐药基因rpoB突变与利福平耐药性的关系。方法采集81例临床标本,分离结核分枝杆菌菌株,PCR扩增rpoB基因及测序,并与利福平药物敏感试验结果比较。结果27例耐利福平菌株中,20株发生rpoB基因突变(74.1%),突变位点包括531和526在内的7个位点10种突变类型,并发现新的突变位点和突变形式;54株敏感株中,1株发生突变(1.9%)。结论利福平耐药rpoB基因突变有一定区域性,为发展快速的基因诊断技术检测耐多药结核病奠定了基础。 |
| 关 键 词： | 结核分枝杆菌 利福平 rpoB基因 突变 药物敏感试验 |
| 收稿时间： | 2009-04-24 |
| 修稿时间： | 2009-06-17 |
Sequencing analysis on rpoB gene mutation of rifampin-resistance in Mycobacterium tuberculosis |
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| GAN Xin,CHEN Ling,WANG Jian-hua,LI Na-na,CHEN Yang,ZHANG Jian-yong,ZHANG Hong. Sequencing analysis on rpoB gene mutation of rifampin-resistance in Mycobacterium tuberculosis[J]. Basic Medical Sciences and Clinics, 2010, 30(3): 268-271 | |
| Authors: | GAN Xin CHEN Ling WANG Jian-hua LI Na-na CHEN Yang ZHANG Jian-yong ZHANG Hong |
| Affiliation: | Dept.of Respiratory Medicine;Affiliated Hospital of Zunyi Medical College;Zunyi 563003;China |
| Abstract: | Objective Genetic evaluation of relationship between rpoB gene mutation and rifampin-resistance of Mycobacterium tuberculosis(MTB).Methods This study was carried out with 81 clinical isolates and sensitivity test and genotypic analysis by PCR amplification and sequencing of rpoB gene were performed.Results Among these isolates,there were 27 rifampin-resistant and 54 sensitive strains.Of the 27 drug-resistant isolates,20 (74.1%) carried mutations on the amplified fragment of the rpoB gene with 10 mutation ty... |
| Keywords: | Mycobacterium tuberculosis rifampin rpoB gene mutation drug susceptibility |
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