妊娠高血压疾病胎盘组织中NGAL和sLox-1的表达及临床意义

目的 分析妊娠高血压疾病孕妇胎盘组织中中性粒细胞明胶酶脂质运载蛋白(NGAL), 凝集素样氧化型低密度脂蛋白受体-1(sLox-1)的变化, 探讨NGAL、sLox-1在妊娠高血压疾病中的临床意义。方法 收集2011年3月~2014年1月在笔者医院确诊为妊娠高血压疾病孕妇68例为实验组, 正常孕妇70例为对照组, 采用免疫组化及Western blot法测定两组胎盘组织中NGAL和sLox-1的表达并进行比较。结果 两组孕妇胎盘组织中均可见NGAL及sLox-1的表达, 但实验组中表达水平明显高于对照组, 两组比较差异有统计学意义(F=43.310, P<0.05);实验组中NGAL及sLox-1的表达随患者病情加重表达愈强, 在子痫前期重度组最明显(F=213.167, P<0.05)。结论 NGAL和sLox-1参与了妊娠高血压疾病病理生理改变, 其发病机制与炎性因子活动有关。

Expression and Clinical Significance of NGAL and sLox-1 in Placenta Tissue of Hypertensive Disorder Complicating Pregnancy

Objective To analyses the changes of neutrophil gelatinase associated lipocalin (NGAL)and lectin-like oxidized low-density lipoprotein receptor-1 (sLox-1) in hypertensive disorder complicating pregnancy placental tissue, and to investigate the clinical significance of NGAL, sLox-1 in pregnancy induced hypertension. Methods Collected the cases in our hospital from March 2011 to January 2014, 68 cases were diagnosed with pregnancy induced hypertension, they were in the experimental group, the 70 cases of normal pregnant women were in the control group. The expression of NGAL and sLox-1 in human placenta were detected by immunohistochemistry and Western blot analysis, then compared their expression in different groups. Results The expression of NGAL and sLox-1 were observed in the two groups of pregnant women placental tissue, but the expression level in the experimental group was significantly higher than that in the control group, there was significant difference between two groups, there was statistically significant (F=43.310, P<0.05). The expression of NGAL and sLox-1 in the experimental group with the sicker patients expressed more strongly, in severe preeclampsia group was most obvious (F=213.167, P<0.05). Conclusion NGAL and sLox-1 participated in the pathophysiology of pregnancy hypertension disease, and its pathogenesis related the inflammatory factor.