可注射骨修复材料结合骨碎补总黄酮修复大鼠颅骨缺损

背景：自体髂骨移植一直被认为是骨缺损修复的“金标准”，但其来源有限。目的：验证应用可注射骨修复材料结合骨碎补总黄酮修复大鼠颅骨缺损的效果。方法：80只雄性SD大鼠建立双侧颅骨缺损模型，随机分为3组：骨修复材料＋骨碎补总黄酮组采用可注射骨修复材料结合骨碎补总黄酮灌胃修复大鼠颅骨缺损：骨修复材料＋去离子水组采用可注射骨修复材料结合去离子水灌胃修复大鼠颅骨缺损；羟基磷灰石＋去离子水组采用羟基磷灰石结合去离子水灌胃修复大鼠颅骨缺损，1次／d，持续8周。于建模后2，4，8周取颅骨标本进行苏木精一伊红染色和Masson染色组织学观察。结果与结论：羟基磷灰石组新骨形成和材料降解速度较慢；可注射骨修复材料组新骨形成和材料降解较羟基磷灰石组快，利于血管及纤维组织长入；骨碎补总黄酮灌胃可以促进血管及纤维组织长入材料，促进成骨。与羟基磷灰石相比，可注射骨修复材料结合骨碎补总黄酮修复大鼠颅骨缺损，可促进新骨形成，缩短骨缺损修复时间。

Injectable bone regeneration composite in combination with assemble flavone of rhizome drynaria repairs rat skull defects

BACKGROUND： Autogenous lilac bone graft has been considered as the ＂gold standard＂ for repair of bone defects, but its resource is limited.OBJECTNE： To analyze the effect of injectable bone regeneration composite （IBRC） combined withassemble flavone of rhizome drynaria （AFDR） in the repair of rat skull defects.METHODS： Eighty male Sprague-Dawley rats were randomly arranged into three groups, and rats in each group were intragastrically administered with IBRC＋AFDR, IBRC＋deionized water, and hydroxyapatite＋deionized water respectively. Treatment was performed once a day, lasting for 8 weeks. Skull samples were taken for hematoxylin-eosin and Masson staining at 2, 4, and 8 weeks after modeling. RESULTS AND CONCLUSION： New bone formation and material degradation were slower in the hydroxyapatite＋ deionized water group than the IBRC＋AFDR and IBRC＋deionized water groups. IBRC was beneficial for the in-growth of vessels and fibers. Intragastdc administration of AFDR could induce bone formation through improving the growth of vessels and fibers into the implant. IBRC＋AFDR can promote bone formation and shorten repair time during the repair of rat skull defects.