骨髓间充质干细胞诱导分化成骨方法的研究与进展

背景：骨髓间充质干细胞具有良好的成骨分化潜能，移植入体内后可分化为骨、软骨、肌肉、脂肪、肝脏和神经等多种细胞。目的：综述大鼠骨髓间充质干细胞诱导分化成骨最新方法进展。方法：分别以“骨髓间充质干细胞，成骨细胞，诱导分化”、“bone mesenchymal stem cells，osteoblast cells，osteogenic differentiation”为检索词，应用计算机检索CHKD期刊全文数据库，PubMed数据库和万方数据库1995至2011年有关文章。纳入有关骨髓间充质干细胞的文献。排除内容重复和缺乏原创性文献。保留34篇文献做进一步分析。结果与结论：骨髓间充质干细胞在适当的生长因子诱导下可以快速地向成骨细胞分化和增殖。在体外诱导骨髓问充质干细胞向成骨细胞分化需要特定的诱导条件、优良的诱导剂以及合适的剂量。骨髓间充质干细胞向成骨细胞分化受许多自身调控因素（控制基因表达的转录因子）和环境调控因素（分泌因素、细胞外基质，化学因素、细胞与细胞间的相互作用）的影响，这些调控因素并不是孤立存在的，而是相互联系、相互作用，共同调控骨髓间充质干细胞向成骨细胞分化。，

Research progress in differentiation of bone marrow mesenchymal stem cells into osteoblasts

BACKGROUND： Bone marrow mesenchymal stem cells have the multi-potent differentiation ability, and can be induced to differentiate into the cells of various tissues including bone, cartilage, muscle, fat, liver and nerve. OBJECTIVE： To review the research progress in induced osteogenic differentiation of bone marrow mesenchymal stem cells. METHODS： We retrieved the articles in CHKD database, PubMed database and Wanfang database published from 1995 to 2011 using the key words ＂bone marrow mesenchymal stem cells, osteoblast cells, osteogenic differentiation＂. Papers concerning bone marrow mesenchymal stem cells were initially included After excluding papers with repeated or obsolete contents, 34 papers were included in the final analysis.RESULTS AND CONCLUSION： Bone marrow mesenchymal stem cells can rapidly differentiate into osteoblasts and proliferate under suitable induction of growth factors. It holds great promise in future application; moreover, it is easy to obtain, separate, expand, and transfect without apparent immune rejection However, there are still some problems such as isolation and purification of bone marrow mesenchymal stem cells, optimal density for differentiation into osteoblasts, oncogenicity, and safety in vivo, which need further investigation.