骨髓间充质干细胞在骨形态发生蛋白2／注射式硫酸钙载体上的成骨

背景：将生长因子女合到支架上构建复合材料可同时具备骨诱导和骨传导作用。目的：脱察骨形态发生蛋白2／注射式硫酸钙复合载体对大鼠骨髓间充质干细胞体外诱导成骨的影响。方法：取生长状态良好的第2代SD大鼠骨髓间充质干细胞悬液，分3组培养：实验组将细胞悬液滴加剑骨形态发生蛋白2，注射式硫酸钙复合材料表面，对照组在细胞悬液中加入骨形态发生蛋白2，空白对照组正常培养。结果与结论：3组细胞随培养时间延长逐渐增多，实验组细胞数量最多，明显多于对照组及空白对照组（P〈0．05）。实验组培养不同时间点碱性磷酸酶活性高于对照组及空白对照组（P〈0．05）。体外实验显示骨形态发生蛋白2，注射式硫酸钙复合载体可促进骨髓间充质干细胞向成骨细胞分化。

In vitro osteogenic induction of bone marrow mesenchymal stem cells on bone morphogenetic protein-2/injectable calcium sulfate carrier

BACKGROUNO：Composite material of growth factor and scaffolds has the function of bone induction and conduction. OBJECTNE： To observe the effect of bone morphogenetic protein-2/injectable calcium sulfate carrier on the in vitro osteogenic induction of bone marrow mesenchymal stem cells.METHODS： The second generation of bone marrow mesenchymal stem cells suspension in good growth state was collected from Sprague Dawley rats, and then divided into three groups. In the experimental group, the cells suspension was dropped on the surface of bone morphogenetic protein-2/injectable calcium sulfate composite; in the control group, bone morphogenetic protein-2 was injected into the cells suspension; cells in the blank control group were cultured normally.RESULTS AND CONCLUSION： The number of the cells in three groups was increased with the prolonging of the incubation time, and the number of the cells in the experimental group was significantly larger than that in the control group and the blank control group （P 〈 0.05）. The alkaline phosphataSe activity at different culturing time points in the experimental group was higher than that in the control group and the blank control group （P 〈 0.05）. In vitro experiment results showed that bone morphogenetic protein-2/injectable calcium sulfate carrier can promote the in vitro osteogenic induction of bone marrow mesenchymal stem cells.