离散因子/肝细胞生长因子cDNA转染对肝癌SMMC7721细胞生长和转移的影响

目的 通过离散因子／肝细胞生长因子（scatter factor/Hepatocyte factor,SF/HGF）cDNA转染肝癌SMMC7721细胞来探讨SF／HGF对肝癌生长和转移的影响。方法 用脂质体法进行基因转染，以ELISA和western blot检测SF／HGF及其受体c-met的表达，通过生长曲线、划良实验比较转染前后细胞的增长状况及细胞的运动能力。以转染前后细胞分别接种裸鼠，观察移植的生长及转移情况。结果 转染后细胞表达SF／HGF的量为694pg/ml，其受体c-met量未见明显变化；转染后细胞增殖明显加快，运动能力增强，并伴明显的形态学变化。初步动物实验显示转染后细胞生长较快，瘤组织中有瘤栓形成，并在肺内发现转移灶。结论 SF／HGF在肝癌细胞中的高表达能促进肝癌细胞生长、侵袭及转移。

Influence of scatter factor/hepatocyte growth factor on the growth and transmission of hepatocarcinoma SMMC 7721 cells

Objective: To explore the influence of scatter factor/hepatocyte growth factor (SF/HGF) on the growth and transmission of hepatocarcinoma SMMC 7721 cells by SF/HGF cDNA transfection. Methods Gene transfection was operated by lipofectin method. In vitro, SF/HGF and c-met expression was tested by ELISA and Western blot. Cell proliferation and motility were compared by growth curves and wound healing assays. In vivo, cells before and after transfection were implanted subcutaneously into inde mice respectively to observe tumor growth and metastasis. Results After transfection, the expression of SF/HGF reached 694 pg/ml, compared with 0.26 pg/ml before transfection; however the expression of c-met protein did not change obviously. Growth curves showed that cells after transfection proliferated more rapidly than those before transfection and had an increased capability of motility together with enlarged morphological changes. In vivo experiment, tumors originated from SF/HGF ( ) cells grew faster than those from SF/HGF (-) cells and had embolism formed inside and metastasis in the corresponding lung tissues, whereas no such findings in SF/HGF (-) cell originated tumors. Conclusions High expression of SF/HGF can stimulate tumor cell proliferation and metastasis in hepatocellular carcinoma.